2007 Fiscal Year Final Research Report Summary
Molecular Biological Study on Partition Mechanism of Chromosome in Escherichia coli
| Project/Area Number |
17570141
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Kyoto University |
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Principal Investigator |
HIRAGA Sota Kyoto University, GRADUATE SCHOOL OF MEDICINE, RESEARCH FELLOW (40027321)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Escherichia coli / plasmid partition / chromosome partition / SecA / SecY / AcpP / MukB / reaction diffusion system |
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| Research Abstract |
The goal of the study is solution of molecular mechanisms on partition of bacterial chromosome and plasmid. We first analyzed cells of Escherichia coli by various cytological techniques and found that replication origin oriC, replication terminus ter, replication forks, and MukB clusters are localized in ordered manner in cell cycle. Furthermore, we examined precisely partition mechanism of F plasmid by immunofluorescence microscopy and FISH. Based on the results, we proposed the new hypothesis that the partition system of F plasmid is a reaction diffusion system of plasmid-coded SopA and SopB. SopA protein might be "activator" and SopB protein might be "inhibitor" in this system. SopA and SopB react each other, resulting in subcellular local positioning of SopB. The sopC region (centromere) of the plasmid binds specifically SopB, and plasmid DNA molecules locate in subcellular positions of high concentration of SopB. Additionally, we found that the partition system of E. coli chromosome is a reaction diffusion system mediated by at least 10 proteins involved SecA, SecY, AcpP and MukB. We named this system "POC (positioning of chromosome) system". In this system, SecA might be "activator" and SecY might be "inhibitor". These proteins react each other and diffuse within the cell, resulting in subcellular local positioning of these proteins. Other proteins of POC subordinate to the dynamics of SecA and SecY. DNA regions that bind specifically DNA binding proteins of POC localize in positions of high concentration of these DNA binding proteins. Thus, subcellular positions of the DNA regions are determined, therefore sister chromosomes are faithfully partitioned into both daughter cells upon cell division.
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[Journal Article] The GTP binding protein Obg homologue ObgE is involved in ribosome maturation.2005
Author(s)
Sato, A., Kobayashi, G., Hayashi, H., Yoshida, A., Wada, A., Maeda, M., and Hiraga, S.
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Journal Title
Molecular Genetics and Genomics 274
Pages: 264-271
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] The GTP binding protein Obg homologue ObgE is involved in ribosome maturation2005
Author(s)
Sato, A., Kobayashi, G., Hayashi, H., Yoshida, A., Wada, A., Maeda, M., Hiraga, S
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Journal Title
Molecular Genetics and Genomics 274
Pages: 264-271
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] ATP-induced shrinkage of DNA with MukB protein and MukBEF complex of Escherichia coli
Author(s)
N., Chen, A.A., Zinchenko, Y., Yoshikawa, S., Araki, S., Adachi, M., Yamazoe, S., Hiraga, K., Yoshikawa
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Journal Title
Journal of Bacteriology (in press)
Description
「研究成果報告書概要(欧文)」より
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