Analysis of effect for DNA structure by phosphorylation of histone protein

2006 Fiscal Year Final Research Report Summary

• Project/Area Number: 17570145
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Nagasaki University
• Principal Investigator: NAKAGAWA Takeya Nagasaki University, school of medicine, assistant, 大学院医歯薬学総合研究科, 助手 (50363502)
• Co-Investigator(Kenkyū-buntansha): YASUI Kiyoshi Nagasaki University, school of medicine, assistant, 大学院医歯薬学総合研究科, 助手 (50372777)
• Project Period (FY): 2005 – 2006
• Keywords: gene / genome / expression control

Research Abstract

We tried to identify the phosphatase of histone H2A c-terminal and clear the effect of this phospholilation for chromatin structure and cell proliferation. But we could not achieve these purposes. We purified the phophatse activity from cell extract and get some candidate molecules. We made recombinant protein of candidate molecules. But these recombinant proteins had no phophatase activity. Next We made the antibody of candidate molecules and used for western blotting. But the fraction which has phsphatase activity contain no candidate molecules. Hereafter we try to analyze another candidate molecules on same ways and purify the phosphatase from the extract which made another way. During this study, we detected the unique histone acetylase activity. So we will try to identify the this acetylase.