2007 Fiscal Year Final Research Report Summary
Environment Responding Regulation of Metamorphosis in the coral Acropora
| Project/Area Number |
17570173
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Ochanomizu University |
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Principal Investigator |
HATTA Masayuki Ochanomizu University, Graduate School of Humanities and Sciences, Associated Professor (00249947)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Coral / Metamorphosis / Marine Bacteria / EcoDevo / Environment Responding |
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| Research Abstract |
Biofilms on submarine substrata can act as efficient inducers for settlement and metamorphosis of Acropora larvae. Indeed, bacteria that induce metamorphosis have been identified from biofilms; one strain of Pseudoalteromonas and three of Alteromonas. Since micro-environments on substrata are very diverse, there could be more bacteria species capable of inducing metamorphosis. Such bacteria were screened by a new method in this study. Each bacteria source was swabbed from a 1 cm^2 surface of tiles submerged in a reef for 3 months, and suspended in sterilized seawater (SSW). Membrane filters were soaked in each suspension and incubated on agar media. For passage, the bacteria-grown filter (mixed culture filter; MCF) was broken into pieces in SSW. We tested each MCF for the activity to induce metamorphosis. Out of 32 MCF lineages from 16 sources, metamorphosis-inducing batches were obtained in 11 from 8. Although individual batches varied in their activities, every lineage contained one or more batches giving 100% metamorphosis. Some lineages retained their activities through passages. Next, a total of 230 independent strains were isolated from 12 active MCFs, and 19 isolates turned out to induce metamorphosis, including 17 giving 100% metamorphosis. According to 16SrDNA sequencing analyses, 17 isolates, except 2 that failed to be identified, fell into 7 species in 4 genera; Pseudoalteromonas, Alteromonas, Vibrio and one in alpha-proteobacteria. Our original culture method using MCFs enabled us to get metamorphosis-inducing bacteria very efficiently, compared with the conventional isolation method (1/80 and 3/500 isolates). This study reveals that inducer bacteria spread over in wide taxa. In addition, we found two Pseudoalteromonas species that inhibit metamorphosis. These findings may suggest very high diversity of bacteria concerned with the metamorphosis decision of Acropora larvae.
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Research Products
(32 results)
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[Journal Article] The application of sexual coral recruits for the sustainable management of ex situ populations in public aquariums to promotecoral reef conservation-SECORE Project.2006
Author(s)
Petersen D., Laterveer M., van Bergen D., Hatta M., Hebbinghaus R., Janse M., Jones R., Richter U., Ziegler T., Visser G., Schumacher H.
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Journal Title
Aquatic Conservation: Marine and Freshwater Ecosystems 16
Pages: 167-179
Description
「研究成果報告書概要(和文)」より
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[Journal Article] The application of sexual coral recruits for the sustainable management of ex situ populations in public aquariums to promote coral reef conservation-SECORE Project.2006
Author(s)
D., Petersen, M., Laterveer D., van Bergen M., Hatta M., Hebbi R., Jones U., Richter T., Ziegler G., Visser H, Schumacher
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Journal Title
Aquatic Conservation : Marine and Freshwater Ecosystems 16
Pages: 167-179
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Hym-301, a novel peptide, regulates the number of tentacles formedin hydra.2005
Author(s)
Takahashil T., Hatta M., Yum S, Koizumi O., Kobayakawa Y., Geel L., Ohtani M., Fujisawa T., Bode H. R.
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Journal Title
Development 132
Pages: 2225-2234
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Hym-301, a novel peptide, regulates the number of tentacles formed in hydra2005
Author(s)
T., Takahashi M., Hatta S, Yum O., Koizumi Y., Kobayakawa L., Gee M., Ohtani T., Fujisawa H. R., Bode
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Journal Title
Development 132
Pages: 2225-2234
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 進化という動的な認識2006
Author(s)
服田昌之
Organizer
日本サンゴ礁学会第9回大会
Place of Presentation
仙台・斉藤報恩会自然史博物館
Year and Date
2006-11-24
Description
「研究成果報告書概要(和文)」より
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[Presentation] ミドリイシサンゴの初期発生2005
Author(s)
大久保奈弥、山上晋、本川達雄、服田昌之
Organizer
日本動物学会第76回大会
Place of Presentation
つくば・つくば国際会議場
Year and Date
2005-10-06
Description
「研究成果報告書概要(和文)」より
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