2007 Fiscal Year Final Research Report Summary
Origin and Development of the Chordate Traits
| Project/Area Number |
17570183
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Developmental biology
|
|---|
| Research Institution | National Institute for Basic Biology |
|---|
Principal Investigator |
TAKAHASHI Hiroki National Institute for Basic Biology, Division for Morphogenesis, Assistant Professor (40283585)
|
|---|
| Project Period (FY) |
2005 – 2007
|
| Keywords | ascidian / development / evolution / notochord / neuron |
|---|
| Research Abstract |
Formation of the chordate body is accomplished by a complex set of morphogenetic movements including convergent extension of notochord cells. In the urochordate ascidian Ciona intestinalis, Brachyury (Ci-Bra) plays a key role in the formation of the notochord, and more than thirty Ci-Bra-downstream notochord genes have been identified. In the present study, we examined the effects of functional suppression of nine Ci-Bra-downstream notochord genes, which include Ci-PTP (protein tyrosine phosphatase), Ci-ACL (ATP: citratelyase), Ci-prickle, Ci-netrin, Ci-trop (tropomyosin-like), Ci-Noto3, Ci-ASAK (ATP sulfurylase/APS kinase), Ci-ERM (ezrin/redixin/meiosin) and Ci-pellino. When function of first two genes (Ci-PTP and Ci-ACL) was suppressed with specific morpholinos, the notochord cells failed convergent, while functional suppression of Ci-prickle resulted in a failure of intercalation, and therefore the cells in these three types of embryo remained in mid-dorsal region of the embryo. Functional suppression of the next four genes (Ci-netrin, Ci-trop, Ci-Noto3 and Ci-ASAK) resulted in the partial defect of intercalation, and the notochord did not consist of a single row of coin-shaped cells. In addition, when the function of the last two genes (Ci-ERM and Ci-pellino) was suppressed, the embryo showed failure of notochord cell elongation, even though convergent/extension took place normally. These results indicate that many Ci-Bra-downstream notochord genes are involved in convergent/extension of the embryo. The results also suggest that an evolutionary event leading to the formation of a chordate-specific character or notochord was the recruitment of genes required for convergent/extension of embryonic cells.
|
-
-
-
-
[Journal Article] A web-based interactive developmental table for ascidian Cion a intestinalis,including 3D real-image embryo reconstructions:I.from fertilized egg to hatching larva2007
Author(s)
Hotta, K., Mituhara, K., Takahashi, H., Inaba, K., Oka, K., Gojobori, T. & Ikeo, K.
-
Journal Title
Dev.Dynamics. 236
Pages: 1790-1805
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
-
-
[Journal Article] A web-based interactive developmental table for ascidian Ciona intestinalis, including 3D real-image embryo reconstructions : I. from fertilized egg to hatching larva.2007
Author(s)
Hotta, K., Mituhara, K., Takahashi, H., Inaba, K., Oka, K., Gojobori, T., Ikeo, K.
-
Journal Title
Dev. Dynamics. 236
Pages: 1790-1805
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
[Presentation] A web-based interactive developmental table for ascidian Ciona intestinalis, including 3D real-image embryo reconstructions.2007
Author(s)
Hotta, K., Takahashi, H., Inaba, K., Oka, K., Ikeo, K.
Organizer
78th Annual meeting for the Japanese Society of Zoolgists.
Place of Presentation
Hirosaki University
Year and Date
2007-09-20
Description
「研究成果報告書概要(欧文)」より
-
[Presentation] Developmental table for the aascidian Ciona intestinalis using 3D real image embryo:I.From fertilized egg to hactching larva2007
Author(s)
Hotta, K., Mitsuhara, K., Takahashi, H., Inaba, K., Oka, K., Gojobori, T.&Ikeo, K.
Organizer
4th International Tunicate Meeting
Place of Presentation
Villefranche,FRANCE
Year and Date
2007-06-26
Description
「研究成果報告書概要(和文)」より
-
[Presentation] Developmental table for the aascidian Ciona intestinalis using 3D real image embryo : I. From fertilized egg to hactching larva.2007
Author(s)
Hotta, K., Mitsuhara, K., Takahashi, H., Inaba, K., Oka, K., Gojobori, T., Ikeo, K.
Organizer
4th International Tunicate Meeting.
Place of Presentation
FRANCE
Year and Date
2007-06-26
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
[Presentation] Functional analysis of Notochord-specific novel genes in the Ciona intestinalis embryos.2006
Author(s)
Yamada, S., Hotta, K., Ueno, N., Satoh, N., Takahashi, H.
Organizer
5th symposium of Development, Differentiation and Regeneration.
Place of Presentation
Kokuyo Hall
Year and Date
2006-12-15
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
-
-
-
-
-
-
-
