2006 Fiscal Year Final Research Report Summary

Studies on trichothecene toxin biosynthesis genes of Fusarium species

Research Project

Project/Area Number	17580043
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Plant pathology
Research Institution	RIKEN
Principal Investigator	KIMURA Makoto RIKEN, RIKEN, Environmental Molecular Biology Laboratory, Senior (20261167)
Project Period (FY)	2005 – 2006
Keywords	Fusarium head blight / trichothecenes / nivalenol / Fusarium graminearum / biosynthesis genes / chemotype / food safety / secondary metabolism
Research Abstract	Trichothecenes are large family of sesquiterenoid secondary metabolites of Fusarium species (e.g., F graminearum) and other molds. f They are major mycotoxins that can cause serious problems when consumed via contaminated cereal grains. In the past 20 years, an outline of trichothecene biosynthetic pathway has been established based on results of precursor feeding experiments and blocked mutant analyses. Following the isolation of the pathway gene Tri5 encoding the first committed enzyme trichodiene synthase, ten biosynthesis genes (Tri genes; two regulatory genes, seven pathway genes, and one transporter gene) were functionally identified in the Tri5 gene cluster. At least three pathway genes, Tri101 (separated alone) and Tril and Tri16 (located in the Tri1- Tri16two- gene cluster), were found outside of the Tri gene cluster. In this study, we searched for genes involved in the biosynthesis of trichothecenes, focusing on those involved in deacetylation of trichothecene skeleton.

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