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2006 Fiscal Year Final Research Report Summary

Function and Role of I gene concerning carotenoid transport

Research Project

Project/Area Number 17580049
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied entomology
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

TSUCHIDA Kozo  National Institute of Infectious Diseases, Division of Radiological Protection and Biology, Chief, 放射線管理室, 室長 (40231435)

Co-Investigator(Kenkyū-buntansha) FUJIMOTO Hirohumi  National Institute of Infectious Diseases, Division of Radiological Protection and Biology, Senior Researcher, 放射線管理室, 主任研究官 (60373396)
Project Period (FY) 2005 – 2006
KeywordsBomby mori / Lutein / Carotenoid-binding protein / Mutant / Yellow blood gene / White blood gene / Transgenic
Research Abstract

The cDNA clone of CBP was obtained from the cDNA library of silk gland using anti-CBP antibody. The deduced amino acid sequence revealed that CBP is a novel member of the steroidogenic acute regulatory (StAR) protein family with its unique structural feature of a StAR-related lipid transfer (START) domain, known to aid in intracellular lipid transfer and recognition. These data suggest that CBP is a new member of the StAR protein family that binds carotenoids rather than cholesterol. CBP is expected to bind and shuttle carotenoids as an intracellular transporter and be one of the components that may make possible to tissue specific delivery of carotenoids. We obtained the evidence that the Y gene corresponds to the CBP gene. Restriction fragment length polymorphism (RFLP) mapping with a cDNA probe of the CBP gene revealed that the Y gene is on the same chromosome with the CBP gene. In the +^Y allele strain, an insertion of a retrotransposon and a partial ORF deletion occurred in the CBP gene locus. The absence of an exon, likely due to an incorrect mRNA splicing caused by the retrotransposon-associated genomic deletion, generates a nonfunctional CBP mRNA lacking the true start methionine. Consequently, CBP protein was only expressed in the Y allele strain. These data indicate that the CBP gene of the +^Y allele is a null allele, suggesting that the Y gene corresponds directly to the CBP gene. Transgenic expression of the CBP gene into a colorless hemolymph strain restored the yellow hemolymph and cocoons, thus verifying the function of CBP as the Y gene product.

  • Research Products

    (14 results)

All 2007 2006 2005

All Journal Article (14 results)

  • [Journal Article] Structural analysis of base mispairing in DNA containing oxidative guanine lesion2007

    • Author(s)
      Fujimoto H., Pinak M., Nemoto T., Bunta JK
    • Journal Title

      CEJP 5

      Pages: 40-61

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Carotenoid silk coloration is controlled by a carotenoid-binding protein, a product of Yellow blood gene2007

    • Author(s)
      Sakudoh T., Sezutsu H., Nakashima T., Kobayashi I., Fujimoto H., Uchino K., Banno Y., Iwano H., Maekawa H., Tamura T., Kataoka H., Tsuchida K.
    • Journal Title

      Proc. Natl. Aca. Sci. USA 104

      Pages: 8941-8946

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mapping Analysisi of carotenoid-binding protein of Bombyx mori by restriction fragment length polymorphism.2007

    • Author(s)
      Hara W., Sosnicki S., Banno Y., Fujimoto H., Takada N., Maekawa H., Fujii H., Wells MA, Tsuchida K.
    • Journal Title

      J. Insect Biotech. Seric. (In press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mechanism of tissue specificity of carotenoids delivery in the silkworm.2007

    • Author(s)
      Sakudoh T., Takada N., Fujimoto H., Tsuchida K.
    • Journal Title

      Carotenoid Sci. (In press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Carotenoid silk coloration is controlled by a carotenoid-binding protein, a product of Yellow blood gene2007

    • Author(s)
      Sakudoh T., Sezutsu H., Nakashima T., Kobayashi I., Fujimoto H., Uchino K., Banno Y., Iwano H., Maekawa H., Tamura T., Kataoka H., Tsuchida K.
    • Journal Title

      Proc. Natl. Aca. Sci. USA. 104

      Pages: 8941-8946

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Mapping Analysis of carotenoid-binding protein of Bombyx mori by restriction fragment length polymorphism.2007

    • Author(s)
      Hara W., Sosnicki S., Banno Y., Fujimoto H., Takada N., Maekawa H., Fujii H., Wells MA, Tsuchida K.
    • Journal Title

      J.Insect Biotech. Seric. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Mechanism of tissue specificity of carotenoids delivery in the silkworm.2007

    • Author(s)
      Sakudoh T., Takada N., Fujimoto H., Tsuchida K.
    • Journal Title

      Carotenoid Sci. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Lipophorin receptor from the silkworm, Bombyx mori : cDNA cloning, genomic structure, alternative splicing and isolation of new isoform.2006

    • Author(s)
      Gopalapillai R., Okuda K., Tsuchida K., Yamamoto K., Nohata J., Ajumura M., Mita K.
    • Journal Title

      J. Lipid Res. 47

      Pages: 1005-1013

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Proteome analysis of silk gland proteins from the silkworm, Bombyx mori2006

    • Author(s)
      Zang P., Aso Y., Yamamoto K., Banno Y., Wang Y., Tsuchida K., Kawaguchi Y., Fujii H.
    • Journal Title

      Proteomics 6

      Pages: 2586-2599

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A novel indicator for radiation sensitivity using the wing size reduction of Bombyx mori pupae caused by g-ray irradiation2006

    • Author(s)
      Takada N., Yamauchi E., Fujimoto H., Banno Y., Tsuchida K., Hashido K., Nakajima Y., Tu Z., Takahashi M., Fujii M., Fugo H., Maekawa H.
    • Journal Title

      J. Insect Biotech and Seric. 75

      Pages: 161-165

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Lipophorin receptor from the silkworm, Bombyx mori : cDNA cloning, genomic structure, alternative splicing and isolation of new isoform.2006

    • Author(s)
      Gopalapillai R., Okuda K., Tsuchida K., Yamamoto K., Nohata J., Ajumura M., Mita K.
    • Journal Title

      J.Lipid Res. 47

      Pages: 1005-1013

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A novel indicator for radiation sensitivity using the wing size reduction of Bombyx mori pupae caused by g-ray irradiation2006

    • Author(s)
      Takada N., Yamauchi E., Fujimoto H., Banno Y., Tsuchida K., Hashido K., Nakajima Y., Tu Z., Takahashi M., Fujii M., Fugo H., Maekawa H.
    • Journal Title

      J.Insect Biotech and Seric. 75

      Pages: 161-165

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Bmstartl, a novel carotenoid-binding protein isform from Bombyx mori, is a orthologous to MLN64, a mammalian cholesterol transporter.2005

    • Author(s)
      Sakudoh, T., Tsuchida K., Kataoka H.
    • Journal Title

      Biochim. Biophys. Res. Comm. 336

      Pages: 1125-1135

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Bmstart1, a novel carotenoid-binding protein isoform from Bombyx mori, is a orthologous to MLN64, a mammalian cholesterol transporter.2005

    • Author(s)
      Sakudoh T., Tsuchida K., Kataoka H.
    • Journal Title

      Biochim.Biophys.Res.Comm. 336

      Pages: 1125-1135

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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