2006 Fiscal Year Final Research Report Summary
Function and Role of I gene concerning carotenoid transport
| Project/Area Number |
17580049
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
TSUCHIDA Kozo National Institute of Infectious Diseases, Division of Radiological Protection and Biology, Chief, 放射線管理室, 室長 (40231435)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Hirohumi National Institute of Infectious Diseases, Division of Radiological Protection and Biology, Senior Researcher, 放射線管理室, 主任研究官 (60373396)
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| Project Period (FY) |
2005 – 2006
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| Keywords | Bomby mori / Lutein / Carotenoid-binding protein / Mutant / Yellow blood gene / White blood gene / Transgenic |
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| Research Abstract |
The cDNA clone of CBP was obtained from the cDNA library of silk gland using anti-CBP antibody. The deduced amino acid sequence revealed that CBP is a novel member of the steroidogenic acute regulatory (StAR) protein family with its unique structural feature of a StAR-related lipid transfer (START) domain, known to aid in intracellular lipid transfer and recognition. These data suggest that CBP is a new member of the StAR protein family that binds carotenoids rather than cholesterol. CBP is expected to bind and shuttle carotenoids as an intracellular transporter and be one of the components that may make possible to tissue specific delivery of carotenoids. We obtained the evidence that the Y gene corresponds to the CBP gene. Restriction fragment length polymorphism (RFLP) mapping with a cDNA probe of the CBP gene revealed that the Y gene is on the same chromosome with the CBP gene. In the +^Y allele strain, an insertion of a retrotransposon and a partial ORF deletion occurred in the CBP gene locus. The absence of an exon, likely due to an incorrect mRNA splicing caused by the retrotransposon-associated genomic deletion, generates a nonfunctional CBP mRNA lacking the true start methionine. Consequently, CBP protein was only expressed in the Y allele strain. These data indicate that the CBP gene of the +^Y allele is a null allele, suggesting that the Y gene corresponds directly to the CBP gene. Transgenic expression of the CBP gene into a colorless hemolymph strain restored the yellow hemolymph and cocoons, thus verifying the function of CBP as the Y gene product.
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[Journal Article] Carotenoid silk coloration is controlled by a carotenoid-binding protein, a product of Yellow blood gene2007
Author(s)
Sakudoh T., Sezutsu H., Nakashima T., Kobayashi I., Fujimoto H., Uchino K., Banno Y., Iwano H., Maekawa H., Tamura T., Kataoka H., Tsuchida K.
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Journal Title
Proc. Natl. Aca. Sci. USA 104
Pages: 8941-8946
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Carotenoid silk coloration is controlled by a carotenoid-binding protein, a product of Yellow blood gene2007
Author(s)
Sakudoh T., Sezutsu H., Nakashima T., Kobayashi I., Fujimoto H., Uchino K., Banno Y., Iwano H., Maekawa H., Tamura T., Kataoka H., Tsuchida K.
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Journal Title
Proc. Natl. Aca. Sci. USA. 104
Pages: 8941-8946
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A novel indicator for radiation sensitivity using the wing size reduction of Bombyx mori pupae caused by g-ray irradiation2006
Author(s)
Takada N., Yamauchi E., Fujimoto H., Banno Y., Tsuchida K., Hashido K., Nakajima Y., Tu Z., Takahashi M., Fujii M., Fugo H., Maekawa H.
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Journal Title
J. Insect Biotech and Seric. 75
Pages: 161-165
Description
「研究成果報告書概要(和文)」より
-
-
[Journal Article] A novel indicator for radiation sensitivity using the wing size reduction of Bombyx mori pupae caused by g-ray irradiation2006
Author(s)
Takada N., Yamauchi E., Fujimoto H., Banno Y., Tsuchida K., Hashido K., Nakajima Y., Tu Z., Takahashi M., Fujii M., Fugo H., Maekawa H.
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Journal Title
J.Insect Biotech and Seric. 75
Pages: 161-165
Description
「研究成果報告書概要(欧文)」より
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