2007 Fiscal Year Final Research Report Summary
Characterization of genes encoding diterpene cyclases responsible for phytoalexin biosynthesis in rice.
| Project/Area Number |
17580093
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Bioproduction chemistry/Bioorganic chemistry
|
|---|
| Research Institution | Yamagata University |
|---|
Principal Investigator |
TOYOMASU Tomonobu Yamagata University, Department of Bioresouroe Engineering, Associate Professor (60272085)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YAMANE Hisakazu University of Tokyo, Biotechnology Research Center, Professor (80090520)
|
|---|
| Project Period (FY) |
2005 – 2007
|
| Keywords | rice / phytoalexin / biosynthesis / cyclase |
|---|
| Research Abstract |
From rice 14 diterpenoid phytoalexins were isolated; momilactones, phytocassanes, and oryzalexins. We have isolated cDNAs encoding diterpene cyclases responsible for biosynthesis of these phytoalexins; OsCPS2, OsCPS4, OsKSL4, OsKSL7, OsKSL8, OsKSL10. This project was for characterization of the cyclase genes. 1) Gene expression patterns: The expression levels in roots of OsCPS2, OsCPS4, OsKSL4 OsKSL7, and OsKSL8, were higher than those in shoots. The expression levels of OsCPS2, OsCPS4, OsKSL4, and OsKSL7 were drastically increased in the rice fruits from heading stage to flowering stage. Transient assay using green fluorescence protein suggested that these diterpene cyclases are transported thin plastids. 2) Isolation of their homologues: From rice KS like homologue cDNAs, OsKSL5 and OsKSL6, which encode ent-pimara-8,15-diene synthase and ent-kaur-15-ene synthase respectively, were isolated. Two homologue cDNAs of OsCPS2 and OsCPS4 were isolated from wheat, other species in Poaceae. 3) Relationship of rice diterpene phytoalexins with pathogenic tolerance: The expression levels of six genes increased after infection by Magnaporthe grisea. We did not succeed in screening Tos 17-insertion-knock-out mutants and production of knock-down mutants by RNAi. 4) Comparison of OsCPS2 to OsCPS1 that is responsible for gibberellin biosynthesis: The severe dwarf phenotype of mutants lacking in OsCPS1 were rescued by ectopic expression of OsCPS2. This result indicated that regulation of OsCPS1 expression is different from that of OsCPS2 expression. The basic enzymatic properties of OsCPS2 were almost same as those of OsCPS1, such as divalent cation requirement, optimal pH, optimal temperature and kinetic parameter. However, the activity of OsCPS2 was suppressed by both of substrate geranylgeranyl diphosphate and a gibberellin-biosynthesis-inhibitor AMO 1618 less than that of OsCPS1.
|
Research Products
(20 results)
-
-
-
[Journal Article] Diterpene phytoalexins arebiosynthesized in and exuded from roots of rice seedlings.2008
Author(s)
Toyomasu, T., Kagahara, T., Okada, K., Koga, J., Hasegawa, M., Mitsuhashi, W., Sassa, T., and Yamane, H.
-
Journal Title
Biosci. Biotechnol. Biochem 72
Pages: 562-567
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Comparison of the enzymaticproperties of ent-copalyl diphosphate synthases in the biosynthesis ofphytoalexins and gibberellins in rice.2008
Author(s)
Hayashi, Y., Toyomasu, T., Hirose, Y., Onodera, Y., Mitsuhashi, W., Yamane, H., Sassa, T., and Dairi, T.
-
Journal Title
Biosci. Biotechnol. Biochem 72
Pages: 523-530
Description
「研究成果報告書概要(欧文)」より
-
-
[Journal Article] Characterization of a rice gene family encoding type-A diterpenecyclases.2006
Author(s)
Kanno, Y., Otomo, K., Kenmoku, H., Mitsuhashi, W., Yamane, H., Oikawa, H., Toshima, H., Matsuoka, M., Sassa, T. and Toyomasu, T.
-
Journal Title
Biosci. Biotechnol. Biochem 70
Pages: 1702-1710
Description
「研究成果報告書概要(欧文)」より
-
-
-
[Presentation] Expression analysis of diterpene cyclase genes responsible for phytoalexin biosynthesis in rice2008
Author(s)
Hirose, Y., Mitsuhashi, W., Okada, K., Yamane, H. and Toyomasu, T.
Organizer
Annual Meeting of Japan Society for Bioscienece, Biotechnology, and Agrochemistry 2008
Place of Presentation
Nagoya
Year and Date
20080300
Description
「研究成果報告書概要(欧文)」より
-
[Presentation] Two ent-copalyl diphosphate synthases in rice2008
Author(s)
Toyomasu, T., Hirose, Y., Kuroda, M., Hayashi, Y., Dairi, T., Mituhashi, W., Matsuoka, M., Yamane, H., Sassa, T.
Organizer
Annual Meeting of Japan Society for Bioscienece, Biotechnology, and Agrochemistry 2008
Place of Presentation
Nagoya
Year and Date
20080300
Description
「研究成果報告書概要(欧文)」より
-
-
[Presentation] Comparison of enzymatic properties of ent-copalyl diphosphate synthases for biosynthesis of phytoalexins and gibberellins2007
Author(s)
Hayashi, Y., Hirose, Y., Onodera, Y., Mitsuhashi, W., Sassa, T., Dairi, T. and Toyomasu, T.
Organizer
42th Annual Meeting of The Japanese Society for Chemical Regulation of Plants
Place of Presentation
Shizuoka
Year and Date
20071000
Description
「研究成果報告書概要(欧文)」より
-
-
-
[Presentation] Type-B diterpene cyclase genes in wheat2006
Author(s)
Toyomasu, T., Hirose, Y., Kagahara, T., Kanno, Y., Otomo, K., Mitsuhashi, W., Oikawa, H., Toshima, H., Sassa, T.
Organizer
Annual Meeting of Japan Society for Bioscienece, Biotechnology, and Agrochemistry 2006
Place of Presentation
Kyoto
Year and Date
20060300
Description
「研究成果報告書概要(欧文)」より
-
[Presentation] Exudation of phytoalexins from rice roots2006
Author(s)
Kagahara, T., Okada, K., Hasegawa, M., Yamane, H., Mitsuhashi, W., Sassa, T. and Toyomasu, T.
Organizer
Annual Meeting of Japan Society for Bioscienece, Biotechnology, and Agrochemistry 2007
Place of Presentation
Tokyo
Year and Date
20060300
Description
「研究成果報告書概要(欧文)」より
-
-
-
[Presentation] Study on diterpene cyclases in wheat2005
Author(s)
Kagahara, T., Hirose, Y., Kanno, Y., Otomo, K., Mitsuhashi, W., Sassa, T. and Toyomasu, T.
Organizer
40th Annual Meeting of The Japanese Society for Chemical Regulation of Plants
Place of Presentation
Tokyo
Year and Date
20051000
Description
「研究成果報告書概要(欧文)」より
-
[Presentation] Study on diterpene cyclases in rice2005
Author(s)
Kanno, Y., Otomo, K., Okada, K., Yamane, H., Mitsuhashi, W., Sassa, T., and Toyomasu, T.
Organizer
40th Annual Meeting of The Japanese Society for Chemical Regulation of Plants
Place of Presentation
Tokyo
Year and Date
20051000
Description
「研究成果報告書概要(欧文)」より
