2006 Fiscal Year Final Research Report Summary
Dynamic behavior of plant polyphenols in biological membranes
| Project/Area Number |
17580115
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | University of Shizuoka |
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Principal Investigator |
NAKAYAMA Tsutomu University of Shizuoka, School of Food and Nutritional Sciences, Professor (50150199)
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| Co-Investigator(Kenkyū-buntansha) |
KAMIHIRA Miya University of Shizuoka, School of Food and Nutritional Sciences, Research Associate (70405140)
NAITO Akira Yokohama National University, Graduate School of Engineering, Professor (80172245)
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| Project Period (FY) |
2005 – 2006
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| Keywords | catechin / polyphenol / phospholipid / lipid membrane / liposome / NMR / EGCg / bicelle |
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| Research Abstract |
Interaction between tea catechins, such as epicatechin gallate (ECg) and epigallocatechin gallate (EGCg), and isotropic bicelle model lipid membranes was investigated by solution NMR techniques. ^1HNMR measurements provided signals from the B-ring and the galloyl moiety in ECg and EGCg that were obviously shifted, and whose proton T_1 relaxation times were shortened upon interaction of the catechins with the bicelles. These results indicate that the B-ring and the galloyl moiety play an important role in this interaction. Nuclear Overhauser effect spectrometry experiments demonstrated that the B-ring and the galloyl moiety are located near the γ-H in the phospholipid trimethylammonium group. On the basis of these findings, we propose that ECg and EGCg interact with the surface of lipid membranes via the choline moiety.
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