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2006 Fiscal Year Final Research Report Summary

Expression analysis of MADS-box genes in Cryptomeria Japonica And Generation of transgenic plants containing MADS-box gene-RNAi constructs

Research Project

Project/Area Number 17580149
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 林産科学・木質工学
Research InstitutionForest Tree Breeding Center

Principal Investigator

KURITA Mannabu  Forest Tree Breeding Center, National Breeding Division, Researcher (40370829)

Co-Investigator(Kenkyū-buntansha) TANIGUCHI Toru  Forest Tree Breeding Center, National Breeding Division, Head (00360470)
Project Period (FY) 2005 – 2006
KeywordsMADS-box gene / flower bud / knock-out / Cryptomeria japonica
Research Abstract

Cryptomeria japonica is one of the most important Japanese conifer species. But the transfer of foreign genes from GM plants to related plant species by pollen has been cited as an environmental concern. For the purpose of creating male sterile GM Cryptomeria japonica, we attempted to identify genes related to male flower formation. We used a lot of knowledge of molecular mechanism related to flower formation, such as MADS-box. In general, plant species contain a variety of MADS-box genes that play important roles in both the formation of flower meristem and the determination of floral organ identity. We have isolated and characterized 6 MADS-box genes from Cryptomeria japonica using PCR amplification and RACE method. RT-PCR analysis revealed that one of these genes, M8p-1, was expressed stronger in male strobilus and its transcriptional activity was increased according as the development of male strobilus.
To study the function of M8p-1 gene in Cryptomeria japonica, we attempt to generate M8p-1-RNAi transgenic plants. To make the RNAi construct for M8p-1 gene suppression, 309bp M8p-1 gene-specific fragment was used. Here we report that MSp-1-RNAi transgenic plants were produced by Agrobacterium-mediated transformation of embryogenic tissues.

  • Research Products

    (8 results)

All 2006 2005

All Journal Article (2 results) Presentation (6 results)

  • [Journal Article] Cloning and characterization of MADS box genes isolated from Cryptomeria japonica male strobih2006

    • Author(s)
      Manabu Kurita
    • Journal Title

      Plant&Cell Physiology Vol.47

      Pages: S175

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cloning and characterization of MADS box genes Isolated from Cryptomeria japonica male strobili.2006

    • Author(s)
      Manabu Kurita
    • Journal Title

      Plant & Cell Physiology(Supplement) Vol. 47, S175

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] スギの雄花形成に関わる遺伝子群の単離2006

    • Author(s)
      栗田 学
    • Organizer
      第36回林木育種研究発表会
    • Place of Presentation
      独立行政法人林木育種センター
    • Year and Date
      20061107-08
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Characterization of expressed genes associated with male strobilus development in Cryptomeria japonica.2006

    • Author(s)
      Manabu Kurita
    • Organizer
      Forest Tree Breeding Association
    • Place of Presentation
      Forest Tree Breeding Center
    • Year and Date
      20060708-11
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] スギの花芽で発現するMADS-box遺伝子の機能解析2006

    • Author(s)
      栗田 学
    • Organizer
      第47回日本植物生理学会年会
    • Place of Presentation
      筑波大学
    • Year and Date
      20060319-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Functional analysis of MADS-box genes expressed in male flowers, of Cryptomeria japonica.2006

    • Author(s)
      Manabu Kurita
    • Organizer
      The Japanese Society of Plant Physiologists
    • Place of Presentation
      Tsukuba University
    • Year and Date
      20060319-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] スギの花芽で発現するMADS-box遺伝子の機能解析2005

    • Author(s)
      栗田 学
    • Organizer
      第23回日本植物細胞分子生物学会・シンポジウム
    • Place of Presentation
      京都大学
    • Year and Date
      20050805-06
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Functional analysis of MADS-box genes expressed in male flowers of Cryptomeria japonica.2005

    • Author(s)
      Manabu Kurita
    • Organizer
      Japanese Society for Plant Cell and Molecular Biology
    • Place of Presentation
      Kyoto University
    • Year and Date
      20050506-08
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2010-02-04  

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