2006 Fiscal Year Final Research Report Summary
Expression analysis of MADS-box genes in Cryptomeria Japonica And Generation of transgenic plants containing MADS-box gene-RNAi constructs
| Project/Area Number |
17580149
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
林産科学・木質工学
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| Research Institution | Forest Tree Breeding Center |
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Principal Investigator |
KURITA Mannabu Forest Tree Breeding Center, National Breeding Division, Researcher (40370829)
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| Co-Investigator(Kenkyū-buntansha) |
TANIGUCHI Toru Forest Tree Breeding Center, National Breeding Division, Head (00360470)
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| Project Period (FY) |
2005 – 2006
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| Keywords | MADS-box gene / flower bud / knock-out / Cryptomeria japonica |
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| Research Abstract |
Cryptomeria japonica is one of the most important Japanese conifer species. But the transfer of foreign genes from GM plants to related plant species by pollen has been cited as an environmental concern. For the purpose of creating male sterile GM Cryptomeria japonica, we attempted to identify genes related to male flower formation. We used a lot of knowledge of molecular mechanism related to flower formation, such as MADS-box. In general, plant species contain a variety of MADS-box genes that play important roles in both the formation of flower meristem and the determination of floral organ identity. We have isolated and characterized 6 MADS-box genes from Cryptomeria japonica using PCR amplification and RACE method. RT-PCR analysis revealed that one of these genes, M8p-1, was expressed stronger in male strobilus and its transcriptional activity was increased according as the development of male strobilus.
To study the function of M8p-1 gene in Cryptomeria japonica, we attempt to generate M8p-1-RNAi transgenic plants. To make the RNAi construct for M8p-1 gene suppression, 309bp M8p-1 gene-specific fragment was used. Here we report that MSp-1-RNAi transgenic plants were produced by Agrobacterium-mediated transformation of embryogenic tissues.
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