2006 Fiscal Year Final Research Report Summary
Identification of fish larvae collected from Tokyo Bay based on genetic biology
| Project/Area Number |
17580161
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Tokyo University of Marine Science and Technology |
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Principal Investigator |
KOHNO Hiroshi Tokyo University of Marine Science and Technology, Faculty of Marine Science, Professor, 海洋科学部, 教授 (90234707)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAMOTO Takashi Tokyo University of Marine Science and Technology, Faculty of Marine Science, Associate Professor, 海洋科学部, 助教授 (40313390)
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| Project Period (FY) |
2005 – 2006
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| Keywords | larvae and juveniles / taxonomy / Tokyo Bay / gene biology / morphology / Yellowfin goby / Acanthogobius |
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| Research Abstract |
Larvae of two gobiids, Acanthogobius flavimanus and A. lactipes, distributed and abundant in the inner part of Tokyo Bay, are difficult to be identified by their morphological characters ; however, they are distinguished each other by their occurrence seasons, the former occurring in Spring and the latter in Summer. Therefore, in this study, larvae of both the species were tried to be identified by the genetic characters.
Adult fish and larvae/juveniles were collected from the inner part of Tokyo Bay. The adults were identified by their morphological characters. Furthermore, their cytochrome b gene of mitochondrial DNA, amplified by using the polymerase chain reaction (PCR), was analyzed by RFLP method, and the nucleotide sequences were obtained.
The larvae were, on the other hand, divided into two types, Type A occurred in Spring and Type B in Summer, and the same genetic analyses were done as in the adults. Consequently, 99.9% of nucleotide sequences of these larvae were matched with those of A. flavimanus and A. lactipes, respectively; therefore, Type A and B larvae were identified to be the respective species. Detailed study on morphology, especially melanophore distribution of these larvae, revealed that the distribution patterns of melanophores on the isthmus and lateral part of body were different between these two species.
The methods performed in this study such as larval collection, larval identification by genetic characters and detailed morphological studies of larvae can be applied widely to the field of larval taxonomy.
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