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2007 Fiscal Year Final Research Report Summary

Diagnosis of uterine involution by assessment of extra-cellular matrices in the cow

Research Project

Project/Area Number 17580284
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Clinical veterinary science
Research InstitutionNational Institute of Agrobiological Sciences

Principal Investigator

KOICHI Ushizawa  National Institute of Agrobiological Sciences, Biology Research Unit, Researcher (90399428)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Toru  National Institute of Agrobiological Sciences, Biology Research Unit, Researcher (20355738)
HASHIZUME Kazuyoshi  Iwate University, Faculty of Agriculture, Professor (10355737)
Project Period (FY) 2005 – 2007
Keywordsbovine / collagen / 7S / uterus / extracellular matrix / immunoassay
Research Abstract

The type IV collagen is the major component of basement membrane and consisted with triplet fibers of collagen molecules. Collagen IV forms supramolecular structure to serve as the scaffold for tissue structure and other basement membrane components. Collagen N comprised three distinct structural domains: 7S domain at the amino terminus; the triple helical domain in the intermediate region; and non-collagenous domain in the carboxyl terminus. Type N collagens associate in a head-to-head fashion to form dimers and in tail-to-tail fashions to form tetramers, to compose sheet-like structure. Among these domains of type N collagen, 7S domains are resistant to collagenases and persistent in the peripheral circulation following matrix remodeling. The concentration of circulating 7S domain could be a good marker for assessment of the tissue remodeling. Bovine uterus dramatically involutes following calving. In the present study, we prepared bovine type N collagen 7S domain and its antiserum for establishing the assessment procedure for uterine involution in the cow. The 7S domain was prepared from bovine placenta. Placental tissues were minced and treated with 8M urea to remove soluble proteins. Residual sediment was collected and used for collagenase digestion. Collagenase digest was chromatographed with Sephacryl S-200HR to isolate high molecular components containing the dodecamer of 7S domain. Isolated fractions were reduced and separated with preparative electrophoresis to collect the monomer of 7S domain. Purified 7S domain was used for antibody generation. In summary we generated bovine type N collagen 7S domain and its antiserum for establishing immunoassay system for measurement of circulating 7S domain concentrations.

  • Research Products

    (26 results)

All 2008 2007 2006 2005

All Journal Article (22 results) (of which Peer Reviewed: 11 results) Presentation (4 results)

  • [Journal Article] Bovine prolactin-related protein-I is anchored to the extracellular matrix through interactions with type IV collagen.2008

    • Author(s)
      Takahashi T, Yamada O, Soares MJ, Hashizume K.
    • Journal Title

      J Endocrinol. 196

      Pages: 225-234

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Hashizume K. Bovine prolactin-related protein-I is anchored to the extracellular matrix through interactions with type IV collagen.2008

    • Author(s)
      Takahashi T, Yamada O, Soares MJ
    • Journal Title

      J Endocrinol. 196(2)

      Pages: 225-34

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Expression and characterization of novel ovine orthologs of bovine placental prolactin-related proteins2007

    • Author(s)
      Ushizawa K, Takahashi T, Hosoe M, Ohkoshi K, Hashizume K.
    • Journal Title

      BMC Mol Biol. 8(論文番号)

      Pages: 95

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Global gene expression analysis and regulation of the principal genes expressed in bovine placenta in relation to the transcription factor AP-2 family2007

    • Author(s)
      Ushizawa K, Takahashi T, Hosoe M, Ishiwata H, Kaneyama K, Kizaki K, Hashizume K.
    • Journal Title

      Reprod Biol Endocrinol. 5(論文番号)

      Pages: 17

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Gene expression and maintenance of pregnancy in bovine : roles of trophoblastic binucleate cell-specific molecules2007

    • Author(s)
      Hashizume K, Ushizawa K, Patel OV, Kizaki K, Imai K, Yamada O, Nakano H, Takahashi T
    • Journal Title

      Reprod Fertil Dev. 19

      Pages: 79-90

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Gene expression profiles of novel caprine placental prolactin-related proteins similar to bovine placental prolactin-related proteins2007

    • Author(s)
      Ushizawa K, Takahashi T, Hosoe M, Kizaki K, Abe Y, Sasada H, Sato E, Hashizume K.
    • Journal Title

      BMC Dev Biol. 7(論文番号)

      Pages: 16

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Biology of the prolactin family in bovine placenta. I. Bovine prolactin-related protein I : Expression, structure and proposed roles2007

    • Author(s)
      Ushizawa K. Hashizume K
    • Journal Title

      Animal Science Journal 77

      Pages: 17-27

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Expression and characterization of novel ovine orthologs of bovine placental prolactin-related proteins.2007

    • Author(s)
      Ushizawa K, Takahashi T, Hosoe M, Ohkoshi K, Hashizume K.
    • Journal Title

      BMC Mol Biol

      Pages: 8-95

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Global gene expression analysis and regulation of the principal genes expressed in bovine placenta in relation to the transcription factor AP-2 family.2007

    • Author(s)
      Ushizawa K, Takahashi T, Hosoe M, Ishiwata H, Kaneyama K, Kizaki K, Hashizume K.
    • Journal Title

      Reprod Biol Endocrinol.

      Pages: 5-17

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Gene expression and maintenance of pregnancy in bovine : roles of trophoblastic binucleate cedd-specific molecules.2007

    • Author(s)
      Hashizume K, Ushizawa K, Patel OV, Kizaki K, Imai K, YAMADA O, Nakano H, Takahashi T.
    • Journal Title

      Reprod Fertil Dev. 19(1)

      Pages: 79-90

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Gene exporession profiles of novel caprine placental prolactin-related proteins to bovine placental prolavtin-related proteins.2007

    • Author(s)
      Ushizawa K, Takahashi T, Hosoe M, Kizaki K, Abe Y, Sasada H, Sato E, Hashizume K.
    • Journal Title

      BMC Dev Biol.

      Pages: 7-16

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Biology of the prolactin family in bovine placenta. I. Bovine placental lactogen : Expression, structure and proposed roles2006

    • Author(s)
      Takahashi T
    • Journal Title

      Animal Science Journal 77

      Pages: 10-17

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Developmental aberrations of liver gene expression in bovine fetuses derived from somatic cell nuclear transplantation2006

    • Author(s)
      Herath CB, Ishiwata H, Shiojima S, Kadowaki T, Katsuma S, Ushizawa K, Imai K, Takahashi T, Hirasawa A, Takahashi S, Izaike Y, Tsujimoto G, Hashizume K
    • Journal Title

      Cloning Stem Cells 8

      Pages: 79-95

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Bovine trophoblast cell culture systems : a technique to culture bovine trophoblast cells without feeder cells2006

    • Author(s)
      Hashizume K, Shimada A, Nakano H, Takahashi T
    • Journal Title

      Methods Mol Med. 121

      Pages: 179-188

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Biology of the prolactin family in bovine placenta. I. Bovine placental lactogen : Expression, structure and proposed roles.2006

    • Author(s)
      Takahashi T
    • Journal Title

      Animal Science Journal. 77

      Pages: 10-17

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Biology of the prolactin family in bovine placenta. I. Bovine prolactin-related protein I : Expression, structure and proposed roles.2006

    • Author(s)
      Ushizawa K. Hashizume K.
    • Journal Title

      Animal Science Journal. 77

      Pages: 18-27

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Developmental aberrations of liver gene expression in bovine fetuses derived from somatic cell nuclear transplantation.2006

    • Author(s)
      Herath CB, Ishiwata H, Shiojima S, Kadowaki T, Katsuma S, Ushizawa K, Imai K, Takahashi T, Hirasawa A, Takahashi S, Izaike Y, Tsujimoto G, Hashizume K.
    • Journal Title

      Cloning Stem Cells. 8(2)

      Pages: 79-95

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Bovine trophoblast cell culture systems : a technique to culture bovine trophoblast cells without feeder cells.2006

    • Author(s)
      Hashizume K, Shimada A, Nakano H, Takahashi T.
    • Journal Title

      Methods Mol Med. 121

      Pages: 179-88

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cloning and expression of two new prolactin-related proteins, prolactin-related protein-VIII and-IX, in bovine placenta2005

    • Author(s)
      Ushizawa K, Takahashi T, Hosoe M, Kaneyama K, Hashizume K
    • Journal Title

      Reprod Biol Endocrinol. 7(論文番号)

      Pages: 68

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] The cytoplasmic expression of E-cadherin and beta-catenin in bovine trophoblasts during binucleate cell differentiation. Placenta2005

    • Author(s)
      Nakano H, Shimada A, Imai K, Takahashi T, Hashizume K
    • Journal Title

      Placenta 26

      Pages: 393-401

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Cloning and expression of two new prolactin-related proteins, prolactin-related protein-VIII and -IX, in bovine placenta.2005

    • Author(s)
      Ushizawa K, Takahashi T, Hosoe M, Kaneyama K, Hashizume K.
    • Journal Title

      Reprod Biol Endocrinol.

      Pages: 3-68

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The cytoplasmic expression of E-cadherin and beta-catenin in bovine trophoblasts during binucleate cell differentiation.2005

    • Author(s)
      Nakano H, Shimada A, Imai K, Takahashi T, Hashizume K.
    • Journal Title

      Placenta. 26(5)

      Pages: 393-401

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Diversity of bovine prolactin-related proteins : expression and localization of new family2006

    • Author(s)
      Ushizawa K, Takahashi T, Hosoe M, Kaneyama K, Hashizume K
    • Organizer
      7th Ruminant reproduction symposium
    • Place of Presentation
      Wellington NZ.
    • Year and Date
      20060800
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] ウシ胎盤組織からのIV型コラーゲン7Sドメインの抽出とその特性について2006

    • Author(s)
      高橋 透、牛澤浩一、細江実佐
    • Organizer
      日本畜産学会大会 2006年3月
    • Place of Presentation
      九州大学
    • Year and Date
      20060300
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] ウシ胎盤性プロラクチンファミリータンパク質;構造・発現動態および機能2006

    • Author(s)
      高橋 透
    • Organizer
      東日本家畜受精卵移植技術研究会シンポジウム招待講演
    • Place of Presentation
      福井県
    • Year and Date
      20060100
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] ウシ栄養膜二核細胞におけるBcl-2ファミリー遺伝子の発現2005

    • Author(s)
      牛澤浩一、高橋 透、細江実佐、金山佳奈子、橋爪一善
    • Organizer
      日本繁殖生物学会大会 2005年9月
    • Place of Presentation
      静岡大学
    • Year and Date
      20050900
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-02-04  

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