2006 Fiscal Year Final Research Report Summary
Analysis of the bacterial flora for the zero emission oriented toilet system and improvement of the clarification function
| Project/Area Number |
17580291
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Boundary agriculture
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
SUZUKI Tomonori Tokyo University of Science, Faculty of Science and Technology, Junior Associate Professor, 理工学部, 講師 (50256666)
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| Project Period (FY) |
2005 – 2006
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| Keywords | zero emission / toilet / septic tank / bacterial flora / PCR-DGGE / 16S rRNA gene / artificial sewage / support |
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| Research Abstract |
The laboratory-scale model zero emission oriented toilet system was operated with the human excrement until 2005. In 2006, the toilet system was operated with the artificial sewage which consisted of peptone, yeast extract, cellulose and NaCl instead of the human excrement, because the components of the human excrement daily changed. And the wood chips or the sponge cubes were packed in a treatment tank as bacterial supports. The results were that the removal of BOD in the wood chips and sponge cubes was 83 and 67%, respectively. The wood chips were necessary to replenish, since the chips were digested by bacteria. The changing the direction of the flow tract was observed in the sponge cubes tank, so it could be considered the reason of less BOD removal.
In 2006, the treatment tank was improved to two levels of aerobic tank and anaerobic tank that filled up sponge cubes. The BOD removal rates in this system almost exceeded 90%. The bacterial flora of the aerobic and anaerobic tank was analyzed, respectively. The results of the bacterial flora and the measurement of the BOD were that the bacteria in the phylum Bacteroidetes carried a main role of the BOD removal in the aerobic tank, and that the bacteria in the phylum Bacterioidetes and the class Betaproteobacteria carried a main role of the BOD removal in the anaerobic tank.
I am going to perform FISH method and quantitive PCR to analyze a change of bacterial quantity to affect the BOD removal suggested by this study in future.
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