Development of glycoddrug using antigenic oligosaccharides that regulate the production of cytokins

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 17580300
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Okayama University
• Principal Investigator: KIMURA Yoshinobu Okayama University, Dept. of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Professor (70195387)
• Project Period (FY): 2005 – 2007
• Keywords: pollen allergen / plant glycoprotein / antigenic oligosaccharide / plant N-glycan / tumor marker oligosaccharide

Research Abstract

The chemical structures of strong antigenic N-glycans perpared from three major pollen allergens, Cry j 1 (Japanese cedar pollen allergen), Jun a 1 (Mountain cedar pollen allergen), and Cha o 1 (Japanese cypress pollen allergen). In the case of Cry j 1 and Jun a 1, we found that Lewis a epitope (Galβ1-3 (Fucα1-4) GlcNAc) occurs in the N-glycan moieties, in addition to the GlcNAc2Man3Xyl1Fuc1GlcNAc2 structure. We analyzed three-dimensional pattern of the antigenic N-glycans occurring ubiquitously on pollen allergens using computer modeling and we found a difference between the 3D pattern of free antigenic N-glycans and that of N-glycans linked to polypeptide-back bone.
We also developed a conjugation method of the antigenic glycopeptides and synthetic polymer (polyglutamic acid) to investigate immunogenic activity of antigenic N-glycans against B-cells and T-cells.
We also found a new tumor antigen oligosaccharide, T-antigen (Galβ1-3Ga1NAc), in royal jelly glycoproteins, which can be used as an anti-tumor glycodrug for antibody production.

Research Products

• [Journal Article] Glycoform analysis of Japanese cypress pollen allergen, Cha o 1: Comparison of glycoform of cedar and cypress pollen allergens (2008)
  • Author(s): Kimura, Y., Kuroki, M., Maeda, M., Okano, M., Yokoyama, M., Kino, K
  • Journal Title: Biosci. Biotechnol. Biochem 72 Pages: 514-522
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Glycoform analysis of Japanese cypress pollen allergen, Cha o 1 : Comparison of glycoform of cedar and cypress pollen allergens (2008)
  • Author(s): Kimura, Y., Kuroki, M., Maeda, M., Okano, M., Yokoyama, M., Kino, K
  • Journal Title: Biosci. Biotechnol. Biochem 72 Pages: 485-491
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Glycoform analysis of Japanese cedar pollen allergen, Cry j 1. (2005)
  • Author(s): Maeda, M., Kamamoto, M., Hino, K., Yamamoto, S., and Kimura, Y
  • Journal Title: Biosci. Biotechnol. Biochem 69 Pages: 1700-17005
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Occurrence of Lewis a epitope in N-glycans of a glycoallergen, Jun a 1, from mountain cedar (Juniperus ashei) pollen. (2005)
  • Author(s): Kimura, Y., Kamamoto, M., Maeda, M., Okano, M., Yokoyama, and M., Kino, K.
  • Journal Title: Biosci. Biotechnol. Biochem 69 Pages: 137-144
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Glycoform analysis of Japanese cedar pollen allergen, Cry j 1 (2005)
  • Author(s): Maeda, M., Kamamoto, M., Hino, K., Yamamoto, S., Kimura, Y
  • Journal Title: Biosci. Biotechnol. Biochem 69 Pages: 1700-17005
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Occurrence of Lewis a epitope in N-glycans of a glycoallergen, Jun a 1, from the mountain cedar pollen (2005)
  • Author(s): Kimura, Y., Kamamoto, M., Maeda, M., Okano, M., Yokoyama, M., Kino, K
  • Journal Title: Biosci. Biotechnol. Biochem 69 Pages: 137-144
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Roles of major carbohydrates on Cry j 1, the major allergen of Cryptomeria japonica pollen, in human IgE and T cell responses (2005)
  • Author(s): Okano, M., Kimura, Y., et. al.
  • Organizer: XVIII th International Symposium on Glycoconjugates
  • Place of Presentation: イタリアフィレンツェ
  • Year and Date: 2005-09-06
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Roles of major carbohydrates on Cry j 1, the major allergen of Cryptomeria japonica pollen, in human IgE and T cell responses (2005)
  • Author(s): Okano, M., Kimura, Y., et. al.
  • Organizer: XVIII th International Symposium on Glycoconjugates
  • Place of Presentation: Florence, Italy
  • Year and Date: 2005-09-06
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] Stuctural and functional features of plant glycoprotein glycans (2008)
  • Author(s): Kimura, Y
  • Total Pages: 61-77
  • Publisher: Comprehensive Glycoscience
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] Comprehensive Glycoscience Vol.3: Structural and functional features of plant glycoprotein glycans (2007)
  • Author(s): Kimura, Y.
  • Total Pages: 17
  • Publisher: Elsevier
  • Description: 「研究成果報告書概要(和文)」より