2006 Fiscal Year Final Research Report Summary
Development of highly sensitive indole and luminol chemiluminescence reagent
| Project/Area Number |
17590034
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | KYUSHU UNIVERCITY |
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Principal Investigator |
NAKAZONO Manabu Kyushu University, Graduate school of pharmaceutical sciences, Research Associate, 大学院薬学研究院, 助手 (90336074)
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| Co-Investigator(Kenkyū-buntansha) |
ZAITSU Kiyoshi Kyushu University, Graduate school of pharmaceutical sciences, Professor, 大学院薬学研究院, 教授 (70091329)
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| Project Period (FY) |
2005 – 2006
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| Keywords | fluorescence / chemiluminescence / bisindolylmaleimide / indole / luminol |
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| Research Abstract |
We synthesized various bisindolylmaleimide (BIM) derivatives, and evaluated their fluorescence (FL) and chemiluminescence (CL) properties. We found BIM derivatives with large Stokes' shifts (200 nm), FL emission maxima wavelengths longer than 500 nm, several FL emissions and a lasting CL (10 min). The possible CL mechanism of BIM was studied by determinating of products after CL reactions using LC-ESI-MS. The results indicated that BIM derivatives emitted via dioxetane of C=C bond at the 2,3-position in maleimide moiety. In this study, computational chemistry was used for theoretical analysis of experimental results. The C=C bond electronic structure and oxidation of C=C bond at the 2,3-position of the maleimide moiety are crucial for producing these FL and CL properties based on our experiment and calculations. This study gives significant facts for development of selective and sensitive luminescence assays with bisindolylmaleimides.
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