2006 Fiscal Year Final Research Report Summary
Study on non-invasive measurement of biological redox for diagnosis of chronic diseases
| Project/Area Number |
17590047
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Sojo University |
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Principal Investigator |
TAKESHITA Keizo Sojo University, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (70175438)
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| Co-Investigator(Kenkyū-buntansha) |
ANZAI Kazunori National Institute of Radiological Sciences, Researcher, 放射線医学総合研究所, 研究員 (70128643)
IKOTA Nobuo National Institute of Radiological Sciences, Researcher, 放射線医学総合研究所, 研究員 (80159649)
MASUMIZU Toshiki Sojo University, Faculty of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (30412737)
OKAZAKI Shoko Sojo University, Faculty of Pharmaceutical Sciences, Research Associate, 薬学部, 助手 (40435152)
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| Project Period (FY) |
2005 – 2006
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| Keywords | Oxidative stress / Redox / ESR / Nitroxyl radical / Imaging |
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| Research Abstract |
1. Acyl-protected hydroxylamine probe (1-acetoxy-3-carbamoyl-2,2,5,5-tetramethylpyrrolidine : ACP) is quickly hydrolyzed in a body to form hydroxylamine, CPH. It is expected that CPH is converted to nitroxyl radical, CP, depending on biological redox status. ESR signal was observed in the upper abdomen after administration of ACP, as measured by ESR imaging system. The signal was durable in both X-ray-irradiated mice and septic mice, while the signal decreased in healthy ones. There was no difference in the total amount of CP and CPH in the liver between healthy and injured mice, indicating that the difference in the duration of ESR signal reflects the difference in the redox status in mice. The duration of ESR signal was also observed in hypertensive mice.
2. New nitroxyl spin probes with spirocyclohexyl rings were designed. The probes were resistant against enzymatic reduction, while their reactivity with ascorbic acid or oxygen radicals was similar to that of probes without spiro-rings.
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[Journal Article] Scandium ion-accelerated scavenging reaction of cumylperoxyl radical by a cyclic nitroxyl radical via electron transfer.2007
Author(s)
Ikuo Nakanishi, Kumiko Kawaguchi, Kei Ohkubo, Tomobori Kawashima, Sushma Manda, Hideko Kanazawa, Keizo Takeshita, Kazunori Anzai, Toshihiko Ozawa, Shunichi Fukuzumi, Nobuo Ikota
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Journal Title
Chem.Lett. 36(3)
Pages: 378-379
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Comparison of Stable Nitroxide, 3-Substituted PROXYLs, with Respect to Protection from Radiation, Prevention of DNA Damage and Distribution in Mice2006
Author(s)
Kazunori Anzai, Megumi Ueno, Akira Yoshida, Masako Furuse, Winn Aung, Ikuo Nakanishi, Takashi Moritake, Keizo Takeshita, Nobuo Ikota
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Journal Title
Free Radic.Biol.Med. 40(7)
Pages: 1170-1178
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Hydroxyl radical generation via photoreduction of a simple pyridine N-oxide by an NADH analogue2005
Author(s)
Ikuo Nakanishi, Chiho Nishizawa, Kei Ohkubo, Keizo Takeshita, Kazuo T.Suzuki, Toshihiko Ozawa, Sidney M.Hecht, Masayuki Tanno, Shoko Sueyoshi, Naoki Miyata, Haruhiro Okuda, Shunichi Fukuzumi, Nobuo Ikota, Kiyoshi Fukuhara
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Journal Title
Org.Biomol.Chem. 3(18)
Pages: 2363-3265
Description
「研究成果報告書概要(欧文)」より
