Mechanisms for adhesion and leakage of pge_2-activated mast cells to and from extracellular matrix

2006 Fiscal Year Final Research Report Summary

• Project/Area Number: 17590079
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Mukogawa Women's University
• Principal Investigator: ICHIKAWA Atsushi Mukogawa Women's University, School of Pharmacy and Pharmaceutical Sciences, Professor, 薬学部, 教授 (10025695)
• Co-Investigator(Kenkyū-buntansha): TANAKA Satoshi Mukogawa Women's University, School of Pharmacy and Pharmaceutical Sciences, Assistant professor, 薬学部, 助教授 (40303846) ; SEMMA Masanori Mukogawa Women's University, School of Pharmacy and Pharmaceutical Sciences, Professor, 薬学部, 教授 (90098533)
• Project Period (FY): 2005 – 2006
• Keywords: cell and tissue / signaling transduction / cell adhesion / mast cells / biological molecules / extracellular matrix

Research Abstract

We obtained following research results during experiment period (2005-2006)
1. A study for adhesion mechanism of PGE_2-activated mast cells: PGE_2 stimulated adhesion of mouse mast cells to plate-bound fibronectin through signaling of EP4 receptor (cAMP-PKA) and EP3 receptor (phospholipase C-Ca_<2+>-calmodulin). The maximum response of EP4 signaling appeared 5-6 h, but that of EP3 15-16 h after stimulation. Down stream signaling was found to be activation of integrin via myosine kinase phosphorylation-dephosphorylation.
2. A study for leakage mechanism of mast cells adhered to fibronectin: The leakage of adhered mast cells to fibronectin, initiated 24 h after PGE2 stimulation, depended on a stimulation of Epac-induced Ca_<2+> mobilization and followed calmodulin activation..
3. PGE_2-receptor EP4-dependent adherence of mast cells to matrix components in subcutaneous tissues around air pouch cavity of mice: We successed to develop a new method for measurement the number of adhered mast cells in subcutaneous tissues and demonstrated the important role of EP4 receptor but not EP3 receptor in the response.

Research Products

• [Journal Article] Activation of histidine decarboxylase through post-translation cleavage by capase-9・in a mouse mastocytoma P-815 (2007)
  • Author(s): Furuta, K., Nakayama, K., Sugimoto, Y., Ichikawa, A., Tanaka, S.
  • Journal Title: J. Biol. Chem. (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Activation of histidine decarboxylase through post-translation cleavage by capase-9 in a mouse mastocytoma P-815 (2007)
  • Author(s): Furuta, K., Nakayama, K., Sugimoto, Y., Ichikawa, A., Tanaka, S.
  • Journal Title: J. Biol. Chem. (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Quantification of the number of EP3 receptors on a living CHO cell surface by the AFM (2006)
  • Author(s): Kim, H., Arakawa, H., Hatae.N., Sugimoto, Y., Ichikawa, A., Narumiya, S.
  • Journal Title: Ultramicroscopy 106 Pages: 652-662
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Membrane orientation of the precursor 74-kDa form of L-histidine decarboxylase (2006)
  • Author(s): Furuta, K., Ichikawa, A., Nakayama, K., Tanaka, S.
  • Journal Title: Inflamm. Res. 55 Pages: 185-191
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Immune regulatory roles of histamine produced by leukocytes (2006)
  • Author(s): Tanaka, S., Ichikawa, A.
  • Journal Title: J. Pharmacol. Sci. 101 Pages: 19-23
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Quantification of the number of EP3 receptors on a living CHO cell surface by the AFM (2006)
  • Author(s): Kim, H., Arakawa, H., Hatae.N., Sugimoto, Y., Ichikawa, A., Narumiya, S.
  • Journal Title: Ultramicroscopy 106(2) Pages: 652-662
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Suppression of allergic Inflammation by the prostaglandin E receptor subtype EP3 (2005)
  • Author(s): Kunikata, T., Yamane, H., Segi, E., Sugimoto, Y., Tanaka, S., Nagai, H., Ichikawa, A., Narumiya, S.
  • Journal Title: Nature Immunology 6 Pages: 524-531
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Prostaglandin E2 stimulates granulocyte colony-stimulating factor production via the prostanoid EP2 receptor in mouse peritoneal neutrophil (2005)
  • Author(s): Sugimoto, Y., Fukuda, Y., Mori, D., Tanaka, S., Yamane, H., Okuno, Y., Ichikawa, A., Tsujimoto, G.
  • Journal Title: J. Immunol. 175 Pages: 2506-2512
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Suppression of allergic inflammation by the prostaglandin E receptor subtype EP3 (2005)
  • Author(s): Kunikata, T., Yamane, H., Segi, E., Sugimoto, Y., Tanaka, S., Nagai, H., Ichikawa, A., Narumiya, S.
  • Journal Title: Nature Immunology 6 Pages: 524-531
  • Description: 「研究成果報告書概要(欧文)」より