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2006 Fiscal Year Final Research Report Summary

Mechanisms for adhesion and leakage of pge_2-activated mast cells to and from extracellular matrix

Research Project

Project/Area Number 17590079
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionMukogawa Women's University

Principal Investigator

ICHIKAWA Atsushi  Mukogawa Women's University, School of Pharmacy and Pharmaceutical Sciences, Professor, 薬学部, 教授 (10025695)

Co-Investigator(Kenkyū-buntansha) TANAKA Satoshi  Mukogawa Women's University, School of Pharmacy and Pharmaceutical Sciences, Assistant professor, 薬学部, 助教授 (40303846)
SEMMA Masanori  Mukogawa Women's University, School of Pharmacy and Pharmaceutical Sciences, Professor, 薬学部, 教授 (90098533)
Project Period (FY) 2005 – 2006
Keywordscell and tissue / signaling transduction / cell adhesion / mast cells / biological molecules / extracellular matrix
Research Abstract

We obtained following research results during experiment period (2005-2006)
1. A study for adhesion mechanism of PGE_2-activated mast cells: PGE_2 stimulated adhesion of mouse mast cells to plate-bound fibronectin through signaling of EP4 receptor (cAMP-PKA) and EP3 receptor (phospholipase C-Ca_<2+>-calmodulin). The maximum response of EP4 signaling appeared 5-6 h, but that of EP3 15-16 h after stimulation. Down stream signaling was found to be activation of integrin via myosine kinase phosphorylation-dephosphorylation.
2. A study for leakage mechanism of mast cells adhered to fibronectin: The leakage of adhered mast cells to fibronectin, initiated 24 h after PGE2 stimulation, depended on a stimulation of Epac-induced Ca_<2+> mobilization and followed calmodulin activation..
3. PGE_2-receptor EP4-dependent adherence of mast cells to matrix components in subcutaneous tissues around air pouch cavity of mice: We successed to develop a new method for measurement the number of adhered mast cells in subcutaneous tissues and demonstrated the important role of EP4 receptor but not EP3 receptor in the response.

  • Research Products

    (9 results)

All 2007 2006 2005

All Journal Article (9 results)

  • [Journal Article] Activation of histidine decarboxylase through post-translation cleavage by capase-9・in a mouse mastocytoma P-8152007

    • Author(s)
      Furuta, K., Nakayama, K., Sugimoto, Y., Ichikawa, A., Tanaka, S.
    • Journal Title

      J. Biol. Chem. (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Activation of histidine decarboxylase through post-translation cleavage by capase-9 in a mouse mastocytoma P-8152007

    • Author(s)
      Furuta, K., Nakayama, K., Sugimoto, Y., Ichikawa, A., Tanaka, S.
    • Journal Title

      J. Biol. Chem. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Quantification of the number of EP3 receptors on a living CHO cell surface by the AFM2006

    • Author(s)
      Kim, H., Arakawa, H., Hatae.N., Sugimoto, Y., Ichikawa, A., Narumiya, S.
    • Journal Title

      Ultramicroscopy 106

      Pages: 652-662

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Membrane orientation of the precursor 74-kDa form of L-histidine decarboxylase2006

    • Author(s)
      Furuta, K., Ichikawa, A., Nakayama, K., Tanaka, S.
    • Journal Title

      Inflamm. Res. 55

      Pages: 185-191

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Immune regulatory roles of histamine produced by leukocytes2006

    • Author(s)
      Tanaka, S., Ichikawa, A.
    • Journal Title

      J. Pharmacol. Sci. 101

      Pages: 19-23

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Quantification of the number of EP3 receptors on a living CHO cell surface by the AFM2006

    • Author(s)
      Kim, H., Arakawa, H., Hatae.N., Sugimoto, Y., Ichikawa, A., Narumiya, S.
    • Journal Title

      Ultramicroscopy 106(2)

      Pages: 652-662

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Suppression of allergic Inflammation by the prostaglandin E receptor subtype EP32005

    • Author(s)
      Kunikata, T., Yamane, H., Segi, E., Sugimoto, Y., Tanaka, S., Nagai, H., Ichikawa, A., Narumiya, S.
    • Journal Title

      Nature Immunology 6

      Pages: 524-531

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Prostaglandin E2 stimulates granulocyte colony-stimulating factor production via the prostanoid EP2 receptor in mouse peritoneal neutrophil2005

    • Author(s)
      Sugimoto, Y., Fukuda, Y., Mori, D., Tanaka, S., Yamane, H., Okuno, Y., Ichikawa, A., Tsujimoto, G.
    • Journal Title

      J. Immunol. 175

      Pages: 2506-2512

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Suppression of allergic inflammation by the prostaglandin E receptor subtype EP32005

    • Author(s)
      Kunikata, T., Yamane, H., Segi, E., Sugimoto, Y., Tanaka, S., Nagai, H., Ichikawa, A., Narumiya, S.
    • Journal Title

      Nature Immunology 6

      Pages: 524-531

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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