2006 Fiscal Year Final Research Report Summary
Biological role for Interferon regulatory factor
| Project/Area Number |
17590084
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
MASUMI Atsuko National Institute of Infectious Diseases, Safety research on blood and biological products, researcher, 血液・安全性研究部, 主任研究官 (70165728)
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| Project Period (FY) |
2005 – 2006
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| Keywords | IRF / transcription / nucleolin / hematopoietic cells / retrovirus / megakaryocyte |
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| Research Abstract |
1) We found that nucleolin binds IRF-2 acetylated by PCAF in culture cells. Nucleolin in the presence of PCAF enhanced IRF-2-dependent H4 promoter activity in NIH3T3 cells. We conclude that nucleolin is recruited to acetylated IRF-2, contributing to transcriptional activation.
2) The effect of IRF-2 on hematopoietic differentiation was investigated. In K562 cells stably tranfected with IRF-2, mRNA level of c-mpl, CD41 were enhanced. In mouse and human CD41 promoter, IRF-2 binding activity was detected and IRF-2 plasmid transfection activated CD41 promoter 2-fold in K562 and UT7/TPO cells. IRF-2 mRNA exists in KSLCD34-cells much higher than other fractionated cells. Colony assay using Methocult and Megacult medium indicated that IRF-2 transduction induced megakaryocyte colonies, not erythroid or macrophage/granulocyte. IRF-2 knockdown by siRNA transfection in mouse stem cells showed the reduction of megakaryocyte differentiation in a colony assay. These results demonstrate that IRF-2 induces the number of megakaryocyte progenitor in mouse stem cells and megakaryocyte differentiation through the enhancement of CD41.
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Research Products
(3 results)
