2007 Fiscal Year Final Research Report Summary
Analysis of the mechanism of cadmium nephropathy based on, cadmium kinetics
| Project/Area Number |
17590106
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental pharmacy
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| Research Institution | Kyoritsu University of Pharmacy |
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Principal Investigator |
TOSHIAKI Shibasaki Kyoritsu University of Pharmacy, Pharmacotherapeutics, Professor (60100921)
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| Co-Investigator(Kenkyū-buntansha) |
HOSOYAMADA Makoto Kyoritsu University of Pharmacy, Pharmaootherapeutics, Associate Professor (00291659)
KIMURA Masaki Kyoritsu University of Pharmacy, Pharmacotherapeutics, Lecturer (40383666)
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| Project Period (FY) |
2005 – 2007
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| Keywords | environment / heavy metals / kidney / toxicology |
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| Research Abstract |
Divalent metal transporter 1 (DMT1) is a mammalian iron (Fe) transporter and also transports Cadmium (Cd) in vitro. This study compared Cd absorption in DMT1-dysfunctional MK/Rej-mk/mk mice (mk/mk mice) and in DMT1-functional, Fe-deficient wild-type (WT) mice, to clarify the role of DMT1 in intestinal Cd absorption in vivo. Mice were given 1 ppm CdCl_2 aq in drinking water for 2 weeks, and the concentrations of Cd and Fe in liver, kidney, and intestinal epithelium were subsequently determined. The Fe concentration in intestinal epithelia of WT mice was decreased in proportion to the level of dietary Fe limitation, while Cd accumulation under the same conditions was increased. DMT1 mRNA expression in the small intestine of Fe-deficient WT mice was dearly increased compared to that in Fe-sufficient WT mice. Iron deficiency resulted in upregulation of Cd uptake in the intestine of Fe-deficient WT mice. The mk/mk mice have a mutation in DMT1 and loss of its function led to decreased intestinal Fe concentration. However, intestinal Cd accumulation was the same as in WT mice and it was also increased in Fe-deficient situation. There is the possibility that an unknown Cd pathway has taken a role on Cd intestinal absorption in vivo and that this pathway is regulated by food Fe concentrations. Therefore, DMT1 is not the sole transporter of intestinal cadmium absorption in viva.
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Research Products
(6 results)
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[Journal Article] Changes of cadmium concentration and expressions of candidates for the cadmium transporter genes in intestine, in short term low dose exposure2008
Author(s)
Tomohito, Suzuki, Makoto, Hosoyamada, Makoto, Sato, Yoko, Namikoshi, Kanae, Momoi, Masaki, Kimura, Toshiaki, Shibasaki
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Journal Title
The Journal of Kyoritsu University of Pharmacy 4
Pages: 1-8
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 鉄欠乏時のDMT1とカドミウム輸送2007
Author(s)
鈴木 友人, 石久 保義紀, 木村 真規, 細山田 真, 柴崎 敏昭
Organizer
フォーラム2007衛生薬学、環境トキシコロジー
Place of Presentation
大阪
Year and Date
20071101-02
Description
「研究成果報告書概要(和文)」より
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