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2006 Fiscal Year Final Research Report Summary

Evaluation and estimation of renal drug elimination based on promoter analyses of organic cation transporters

Research Project

Project/Area Number 17590119
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Medical pharmacy
Research InstitutionMie University

Principal Investigator

OKUDA Masahiro  Mie University, Mie University Hospital, Professor, 医学部附属病院, 教授 (70252426)

Co-Investigator(Kenkyū-buntansha) MIZUTANI Hideki  Mie University Hospital, Lecturer, 医学部附属病院, 講師 (80397504)
Project Period (FY) 2005 – 2006
Keywordsorganic cation transporter / renal proximal tubules / OCT2 / promoter / gender differences / testosterone / transcriptional regulation / basic drugs
Research Abstract

Organic cation transporter OCT2, expressed in the basolateral membranes of renal proximal tubules, is considered to mediates inter-and intra-individual variability of pharmacokinetics of basic drugs. However, mechanism of expressional regulation is scarcely known. In the present study, we analyzed the mechanism of testosterone-mediated regulation of OCT2 expression in detail, using deleted constructs and constructs with mutated AREs.
1. Cloning of promoter regions of OCT1, 2, and 3
Promoter regions of OCT1 and OCT3 were isolated by PCR using rat genomic DNA as a template and specific primers for OCT1 and OCT3. Promoter region of OCT2 was isolated by screening rat genomic library.
2. Stimulation of transcription of OCT2 by testosterone
After insertion of each promoter region into pGL3 vector, it was introduced into LLC-PK1 cells, cultured renal epithelial cells derived from pig kidney, with rat androgen receptor. Promoter activity of OCT2 was stimulated by testosterone at 1 nM and higher, and was inhibited by nilutamide, an antagonist of androgen receptor.
3. Analyses of transcription activity using deleted constructs and constructs with mutated AREs
Promoter activity was analyzed using deleted constructs and constructs with mutated ARE located in the promoter region of OCT2. As the conclusion, two distinct AREs, ARE-1 and ARE-3, were clarified to have relevant roles in the stimulation of transcription activity of OCT2 by testosterone.

  • Research Products

    (18 results)

All 2006 2005

All Journal Article (16 results) Book (2 results)

  • [Journal Article] Androgen receptor is responsible for rat organic cation transporter 2 gene regulation but not for rOCT1 and rOCT3.2006

    • Author(s)
      Asaka, J. et al.
    • Journal Title

      Pharm. Res. 23・ 4

      Pages: 697-704

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Clinical evaluation of plasma free phenytoin measurement. and factors influencing its protein binding.2006

    • Author(s)
      Iwamoto, T. et al.
    • Journal Title

      Biopharm. Drug Dispos. 27・2

      Pages: 77-84

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Interactions of fluoroquinolone antibacterials, DX-619 and levofloxacin, with creatinine transport by renal organic cation transporter hOCT2.2006

    • Author(s)
      Okuda, M. et al.
    • Journal Title

      Drug Metab. Pharmacokinet. 21・5

      Pages: 432-436

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Androgen receptor is responsible for rat organic cation transporter 2 gene regulation but not for rOCT1 and rOCT3.2006

    • Author(s)
      Asaka, J., Terada, T., Okuda, M., Katsura, T., Inui, K.
    • Journal Title

      Pharm. Res. 23-4

      Pages: 697-704

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Clinical evaluation of plasma free phenytoin measurement and factors influencing its protein binding. Biopharm.2006

    • Author(s)
      Iwamoto, T., Kagawa, Y., Naito, Y., Kuzuhara, S., Okuda, M.
    • Journal Title

      Drug. Dispos. 27-2

      Pages: 77-84

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Interactions of fluoroquinolone antibacterials, DX-619 and levofloxacin, with creatinine transport by renal organic cation transporter hOCT2.2006

    • Author(s)
      Okuda, M., Kimura, N., Inui, K.
    • Journal Title

      Drug Metab. Pharmacokinet. 21-5

      Pages: 432-436

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Regulation of human peptide transporter 1 (PEPT1) in gastric cancer cells by anticancer drugs.2005

    • Author(s)
      Inoue, M. et al.
    • Journal Title

      Cancer Lett. 230・1

      Pages: 72-80

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Metformin is a superior substrate for renal organic cation transporter OCT2 rather than hepatic OCT1.2005

    • Author(s)
      Kimura et al.
    • Journal Title

      Drug Metab. Pharmacokinet. 20・5

      Pages: 379-386

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Initial dosage adjustment for oral administration of tacrolimus using the intestinal MDRI level in living-donor liver transplant recipients.2005

    • Author(s)
      Masuda, S. et al.
    • Journal Title

      Transplant. Proc. 37・4

      Pages: 1728-1729

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Modulation of P-glycoprotein expression in hyperthyroid rat tissues.2005

    • Author(s)
      Nishio, N. et al.
    • Journal Title

      Drug Metab. Dispos. 33・11

      Pages: 1584-1587

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Increased protein level of PEPT1 intestinal H+-peptide cotransporter upregulates absorption of glycylsarcosine and ceftibuten in 5/6 nephrectomized rats.2005

    • Author(s)
      Shimizu, Y. et al.
    • Journal Title

      Am. J. Physiol. Gastrointest. Liver Physiol. 288・4

      Pages: G664-G670

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Regulation of human peptide transporter 1 (PEPT1) in gastric cancer cells by anticancer drugs.2005

    • Author(s)
      Inoue, M., Terada, T., Okuda, M., Inui, K.
    • Journal Title

      Cancer Lett. 230-1

      Pages: 72-80

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Metformin is a superior substrate for renal organic cation transporter OCT2 rather than hepatic OCT1.2005

    • Author(s)
      Kimura, N., Masuda, S., Tanihara, Y., Ueo, H., Okuda, M., Katsura, T., Inui, K.
    • Journal Title

      Drug Metab. Pharmacokinet. 20-5

      Pages: 379-386

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Initial dosage adjustment for oral administration of tacrolimus using the intestinal MDR1 level in living-donor liver transplant recipients.2005

    • Author(s)
      Masuda, S., Goto, M., Okuda, M., Ogura, Y., Oike, F., Kiuchi, T., Tanaka, K., Inui, K.
    • Journal Title

      Transplant. Proc. 37-4

      Pages: 1728-1729

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Modulation of P-glycoprotein expression in hyperthyroid rat tissues.2005

    • Author(s)
      Nishio, N., Katsura, T., Ashida, K., Okuda, M., Inui, K.
    • Journal Title

      Drug Metab. Dispos. 33-11

      Pages: 1584-1587

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Increased protein level of PEPT1 intestinal H+-peptide cotransporter upregulates absorption of glycylsarcosine and ceftibuten in 5/6 nephrectomized rats..2005

    • Author(s)
      Shimizu, Y., Masuda, S., Nishihara, K., Ji, L., Okuda, M., Inui, K.
    • Journal Title

      Am. J. Physiol. Gastrointest. Liver Physiol. 288-4

      Pages: G664-G670

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 有機カチオントランスポーターOCTs創薬動態-医薬品創製のための考え方と最新情報2006

    • Author(s)
      奥田真弘
    • Total Pages
      506
    • Publisher
      日本薬物動態学会
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 医療薬学 第4版2005

    • Author(s)
      奥田真弘
    • Total Pages
      419
    • Publisher
      廣川書店
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2008-05-27  

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