2006 Fiscal Year Final Research Report Summary
Gut like structures and gut tissues from mouse ES cells and their usage for developmental biology
| Project/Area Number |
17590158
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Nagoya University |
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Principal Investigator |
TORIHASHI Shigeko Nagoya University, School of Medicine, Professor, 医学部, 教授 (90112961)
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| Co-Investigator(Kenkyū-buntansha) |
SENDA Takao Fujita Health University, School of Medicine, Professor, 医学部, 教授 (10187875)
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| Project Period (FY) |
2005 – 2006
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| Keywords | ES cell / Gastrointestinal tract / Development / Smooth muscle / Spontaneous contraction / BMP / PDGF / Interstitial cells of Cajal |
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| Research Abstract |
The aims of the project were establishment of the culture system to develop gut-like structures from mouse ES cells as an in vitro developmental model and usage for investigation of gut organogensis. We established the culture system by hanging drops to form embryoid bodies and plating them on gelatin coated dishes to outgrowth. The gut-like structures were analyzed morphologically and molecular biologically. We found that the formation process of gut-like structures mimicked gut organogenesis and transcription factors crucial for the gut development were timely expressed. Therefore, we concluded our system was an in vitro model of gut development. We published data in appropriate journals.
The differentiation mechanism of musculature of the gastrointestinal (GI) tract has been remained to clarify and it has been suggested Bone Morphogenetic Protein (BMP) signaling is involved in the process. We used gut-like structures for analysis of the mechanism and put agarose beads soaked with BMPs near developing gut-like structures. During the smooth muscle differentiation in the gut-like structures, BMPs especially BMP2 advanced the differentiation and formed substantial musculature showing spontaneous rhythmical contractions. Real time PCR revealed some transcription factors relating to smooth muscle differentiation increased after insertion of BMP beads. We are processing the data for publication now.
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