2007 Fiscal Year Final Research Report Summary
Identfication of novel genes highly expreseed in the mitotic neural progenitor/stem cells
| Project/Area Number |
17590173
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Dokkyo Medical University |
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Principal Investigator |
SAKAKIBARA Shin-ichi Dokkyo Medical University, Deparment of Histology & Neurobiology, Associate Professor (70337369)
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| Co-Investigator(Kenkyū-buntansha) |
UEDA Shuichi Dokkyo Medical Univenrity, Department of Histology & Neurobiology, Professor (60150570)
NAKADATE Kazuhiko Dokkyo Medical Universtiy, Department of Histology & Neurobiology, Associate Professor (80372895)
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| Project Period (FY) |
2005 – 2007
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| Keywords | neural stem cell / Radmps / mitotic spindle / microtubules / neural progenitor cell / central nervous system / neurogenesis |
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| Research Abstract |
Identification of the genes regulating neural progenitor neural stem cell functions is critical to understand the mechanisms of the adult neurogenesis and neurodegenerative disease. We compared the gene expression profile of proliferating neural stem cell culture with those of differentiated cells, and identified a novel gene named "Radmis (radial fiber associated mitotic spindle protein)". Northern blot analysis indicated radmis mRNA is expressed in the neural stem cells in vitro. In the developing and matured CNS, radmis protein was highly enriched in the mitotic neural progenitors. At E11 and E13 developing cerebral cortex, radmis expression was observed in the mitotic spindle, and radial fiber of the nestin-positive neuroepithelial cells, neural stem cells, located at the ventricular surface. At early postnatal CNS, radmis is expressed in positive neural progenitor cells in the SVZ of lateral ventricles, and 4th ventricle. In the developing cerebellum at P6, radmis is expressed in
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the mitotic spindle of the proliferating neuronal progenitor cells within the EGL layer, the superficial layer of cerebellum, but it was absent from the NeuN-positive post-mitotic neurons in both EGL arid IGL, the inner layer of the cerebellum. Radmis expression is drastically decreased as the CNS development proceeds, but a small number of cells in SVZ neurogenic region remained to express radmis protein. In the adult SVZ surrounding the lateral ventricles, radmis is expressed in the dividing neural stem cells (type B cells) that are positive for GFAP, and also in the proliferating neuronal progenitors (rapid amplifying cells, type C cells) that are positive for nestin. During the cell cycle, radmis protein did not expressed in the microtubules in interphase cells. At prometaphase, metaphase and anaphase, radmis colocalized with mitotic spindle poles and spindle microtubules proximal to the centrosomes, suggesting that radmis may control the spindle structure and function during the embryonic and adult neurogenesis. Less
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[Journal Article] The RNA-binding protein HuD regulates neuronal cell identity and maturation2005
Author(s)
Akamatsu W, Fujihara H, Mitsuhashi T, Yano M, Shibata S, Hayakawa Y, Okano HJ, Sakakibara S, Takano H, Takana T, Takahashi T, Node T, Okano H
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Journal Title
Proc Natl Acad Sci USA 102
Pages: 4625-4630
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] The RNA-binding protein HuD regulates neuronal cell identity and maturation2005
Author(s)
Akamatsu, W, Fujihara H, Mitsuhashi T, Yano M, Shibata S, Hayakawa Y, Okano, HJ, Sakakibara S, Takano H, Takano T, Takahashi T, Noda T, Okano, H
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Journal Title
Proc Natl Aced Sci USA 102
Pages: 4625-4630
Description
「研究成果報告書概要(欧文)」より
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