Early-detection, diagnosis and monitoring of malignant lymphoma/leukemia.

2006 Fiscal Year Final Research Report Summary

• Project/Area Number: 17590302
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Okayama University
• Principal Investigator: OKA Takashi Grad, Sch.of Med., Den., & Pham. Sci., Assistant Prof., 大学院医歯薬学総合研究科, 助手 (50160651)
• Co-Investigator(Kenkyū-buntansha): YOSHINO Tadashi Grad, Sch.of Med., Den., & Pham. Sci., Prof., 大学院医歯薬学総合研究科, 教授 (70183704) ; OUCIDA Mamoru Grad, Sch.of Med., Den., & Pham. Sci., Associate Prof., 大学院医歯薬学総合研究科, 助教授 (80213635) ; SHINAGAWA Katsuzi Grad, Sch.of Med., Den., & Pham. Sci., Assistant Prof., 医学部・歯学部附属病院, 助手 (00273988)
• Project Period (FY): 2005 – 2006
• Keywords: Epigenetic / SHP1 / Methylation / Leukemia / Lymphoma / CIM P

Research Abstract

There are various kinds of hematopoietic malignancies including lymphoma and leukemia. There is wide range of spectrum in terms of prognosis. Some of them are very aggressive and hard to control. Others show slightly well prognosis. In many cases, relapse will occur after the regression of tumor cells with the treatments of chemotherapy, x-irradiation or immuno-therapy etc., if only a small number of tumor cells could survive. In order to prevent relapse and induce complete remission, development of highly sensitive, specific technology to detect tumor cells is urgently required.
We found highly frequent gene silencing of specific genes with DNA hyper-methylation in hematopoietic malignancies. Especially, we found that some type of malignant lymphoma/leukemia show specific changes of methylation profiles and also CpG island methylator phenotype (CIMP) in accordance with the progression. In the basis of these findings, we developed a new highly sensitive, specific system to detect hematopoietic malignancies. It will be useful for the early-detection, diagnosis and monitoring of malignant lymphoma/leukemia.

Research Products

• [Journal Article] Down-regulation of ASY/Nogo transcription associated with progression of adult T-cell leukemia/lymphoma. (2006)
  • Author(s): Shimakage M., Inoue N., Oka T., et al.
  • Journal Title: Int. J. Cancer 119 Pages: 1648-1653
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] P27Kipl is detected on most gastric MALT lymphomas, but not large cell lymphomas (2006)
  • Author(s): Sato H., Sato Y., Ichimura K., Oka T., Kondo E., Tanaka T., Kondo T., Ohara N., Takahashi K, Yoshino T
  • Journal Title: J. Clin. Experment. Hematol 46(1) Pages: 25-30
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] P27Kip1 is detected on most gastric MALT lymphomas, but not large cell lymphomas (2006)
  • Author(s): Sato H., Sato Y., Ichimura K., Oka T., Kondo E., Tanaka T., Kondo T., Ohara N., Takahashi K., Yoshino T.
  • Journal Title: J. Clin. Experiment. Hematol. 46(1) Pages: 25-30
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] High frequent gene silencing of hematopoietic cell specific protein tyrosine phosphatase SHP1 in hematopoietic cell malignancies. (2006)
  • Author(s): T.Oka, M.Ouchida, M.Tanimoto, K.Shimizu, T.Yoshino
  • Journal Title: Gene silencing : New research (edited by G.W. Redberry) (Nova Science Publishers, Inc.) (New York) Pages: 1-34
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Analysis of genomic homology of murine gammaherpesvirus (MHV)-72 to MHV-68 and impact of MHV-72 on the survival and tumorigenesis in the MHV-72-infected CB17 scid/scid and CB17+/+mice. (2005)
  • Author(s): Oda W, Mistrikova J, Stancekova M, Dutia BM, Nash AA, Takahata H, Jin Z, Oka T, Hayashi
  • Journal Title: Pathol. Int. 55(9) Pages: 558-568
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Expression of phosphorylated Ser70 of Bcl-2 correlates with malignancy in human colorectal neoplasms. (2005)
  • Author(s): Kondo E, Miyake T, Shibata M, Kimura T, Iwagaki H, Nakamura S, Tanaka T, Ohara N, Ichimura K, Oka T, Yanai H, Shibasaki F, Yoshino T
  • Journal Title: Clin Cancer Res. 11(20) Pages: 7255-7263
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Analysis of genomic homology of murine gammaherpesvirus (MHV)-72 to MHV-68 and impact of MHV-72 on the survival and tumorigenesis in the MHV-72-infected CB17 scid/scid and CB17+/+ mice. (2005)
  • Author(s): Oda W, Mistrikova J, Stancekova M, Dutia BM, Nash AA, Takahata H, Jin Z, Oka T, Hayashi.
  • Journal Title: Pathol. Int. Sep;55(9) Pages: 558-68
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of phosphorylated Ser70 of Bcl-2 correlates with malignancy in human colorectal neoplasms. (2005)
  • Author(s): Kondo E, Miyake T, Shibata M, Kimura T, Iwagaki H, Nakamura S, Tanaka T, Ohara N, Ichimura K, Oka T, Yanai H, Shibasaki F, Yoshino T.
  • Journal Title: Clin Cancer Res. Oct 15;11(20) Pages: 7255-63
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Down-regulation of drs mRNA is associated with the progression of adult T-cell leukemia/lymphoma
  • Author(s): Shimakage, M., Inoue, N., Oshima, K., Oka, T., Tambe, Y., Yasui, K., Matsumoto, K., Yutsudo, M., Inoue, H.
  • Journal Title: Int. J. Oncol. (In press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Down-regulation of drs mRNA is associated with the progression of adult T-cell leukemia/lymphoma.
  • Author(s): Shimakage M., Inoue N., Oshima K., Kawahara K., Yamamoto N., Oka T., Tambe Y., Yasui K., Matsumoto K., Yutsudo M., Inoue H.
  • Journal Title: Int. J. Oncol. (In press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] High frequent gene silencing of hematopoietic cell specific protein tyrosine phosphatase SHP1 in hematopoietic cell malignancies. In "Gene silencing : New research". (2006)
  • Author(s): T.Oka., M.Ouchida., M.Tanimoto., K.Shimizu, T.Yoshino.
  • Total Pages: 1-34
  • Publisher: Nova Science Publishers, Inc. (New York)
  • Description: 「研究成果報告書概要(和文)」より