2006 Fiscal Year Final Research Report Summary
Research of the modification of immunological functions of T and NK-cells with Epstein-Barr virus infection
| Project/Area Number |
17590359
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Experimental pathology
|
|---|
| Research Institution | Iwate Medical University |
|---|
Principal Investigator |
KANNO Hiroyuki Iwate Medical University, School of Medicine, Department of Pathology, Associate Professor (40252663)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ENDO Mikiya Iwate Medical University, School of Medicine, Department of Pathology, Lecturer (80254770)
KAMATAKI Akihisa Iwate Medical University, School of Medicine, Department of Pathology, Assistant (60360004)
|
|---|
| Project Period (FY) |
2005 – 2006
|
| Keywords | Epstein-Barr virus(EBV) / cytokines / Chronic active EBV infection / vasculitis / adhesion molecules |
|---|
| Research Abstract |
To evaluate the effects of Epstein-Barr virus (EBV)-encoded small RNAs (EBERs) on the expression of cytokines in human T-cells, the EBER-expressing plasmid was introduced into three human T-cell lines with site-specific recombination, and the expression of mRNAs of various cytokines was examined with real-time RT-PCR analyses. The expression of EBERs caused the alteration of cytokine expression in a cell line-dependent manner. However, the expression of EBERs in these T-cell lines was generally lower than that in EBV-positive NK/T-cell lines, and only one of three cell lines expressed EBERs at the detectable level with Northern blot analyses. In this cell line, the expression of interleukin-10 was up-regulated and the phosphorylation of dsRNA-dependent protein kinase (PKR) was decreased, indicating that EBERs play a role in the cell line.
On the other hand, EBV-latent membrane protein-1 (LMP1)-expressing human T-cell lines were not established with site-specific recombination. Furthermo
… More
re, RNA interference to LMP1 in EBV-positive NK/T-cell lines was not successful. Thus, the role of LMP1 in NK/T-cells was still unclear. LMP1 is, however, still a candidate for the responsible gene of cytokinemia, an important pathophysiology of chronic active EBV infection (CAEBV), because the effects of EBERs on expression of Th1 cytokines were not constant as mentioned above.
Approximately one-fourth of CAEBV patients develop vascular lesions with infiltration of EBV-positive lymphoid cells. EBV-positive NK-lymphoma cell lines, SNKs, produced TNFα, which caused the increased expression of ICAM-1 and VCAM-1 in cultured human endothelial cells. Furthermore, SNKs exhibited the increased adhesion to cultured endothelial cells stimulated with TNFα or interleukin-lβ, and the pre-treatment of these cells with anti-VCAM-1-antibodies reduced the cell adhesion. These indicate that the up-regulated expression of VCAM-1 on cytokine-stimulated endothelial cells would be important for the adhesion of EBV-positive NK-cells and might initiate the vascular lesions. Less
|
-
-
-
[Journal Article] The role of heparin-binding EGF-like growth factor and amphiregulin in the epidern, al proliferation of psoriasis in cooperation with TNFα.2008
Author(s)
Yoshida, A., Kanno, H., Watabe, D., Akasaka, T., Sawai, T.
-
Journal Title
Arch. Dermatol. Res. 300(1)
Pages: 37-45
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
-
[Journal Article] Progressive wild-type transthyretin deposition after liver transplantation preferentially occurs onto myocardium in FAP patients.2007
Author(s)
Yazaki, M., Mitsuhashi, S., Tokuda, T., Kametani, F., Takei, Y.-l., Koyama, J., Kawamorita, A., Kanno, H., Ikeda, S.-I.
-
Journal Title
Am. J. Transplant. 7(1)
Pages: 235-242
Description
「研究成果報告書概要(欧文)」より
-
-
-
[Journal Article] Adhesion of peripheral blood mononuclear cells and CD4'T cells from patients with psoriasis to cultured endothelial cells via the interaction between lymphocyte function- associated antigen type 1 and intercellular adhesion molecule 1.2007
Author(s)
Watabe. D, Kanno, H., Yoshida, A., Kurose, A., Akasaka, T., Sawai, T.
-
Journal Title
Br. J. Dermatol. 157(2)
Pages: 259-265
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
[Journal Article] Downregulation of inhibitor of apoptosis proteins in apoptotic human chondrocytes treated with tumor necrosis factor-alpha and actinomycin P Osteoarthritis and Cartilage2006
Author(s)
Yoshimura, F., Kanno, H., Uzuki, M., Tajima, K., Shimamuta, T., Sawal, T.
-
Journal Title
P Osteoarthritis and Cartilage 14(5)
Pages: 435-441
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
[Journal Article] Vascular lesion in a patient of chronic active Epstein-Barr virus infection with the hypersensitivity to mosquito bites : vasculitis induced by mosquito bite with the infiltration of nonneoplastic Epstein-Barr virus-positive cells and subsequent development of natural killer/T-ceT lymphoma with angiodestruction.2005
Author(s)
Kanno. H. Onodera, H., Erido, M., Maeda, F., Chida, S., Akasaka, T., Sawal, T.
-
Journal Title
Hum. Pathol. 36(2)
Pages: 212-218
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
[Journal Article] Lymphoepithelioma-like carcinoma of the breast. Report of a case with the first electron microscopic study and review of the literature.2005
Author(s)
Kurose, A., Ichinohasama, R., Kanno. H., Kobayashi, T., Ishida, M., Nishinari, N, Sawai, T.
-
Journal Title
Virchows Archiv 447(9)
Pages: 653-659
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
