2006 Fiscal Year Final Research Report Summary
Regulatory mechanisms of production of virulence factor in Vibrio parahaemolyticus
| Project/Area Number |
17590393
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | The University of Tokushima |
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Principal Investigator |
TAKAHASHI Akira The University of Tokushima Graduate School, Institute of Health Biosciences, Associate Professor, 大学院ヘルスバイオサイエンス研究部, 助教授 (90304047)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Hiroshi The University of Tokushima, Institution Genome Research, Associate Professor, ゲノム機能研究センター, 助教授 (20294639)
NAKANO Masayuki The University of Tokushima Graduate School, Institute of Health Biosciences, COE researcher, 大学院ヘルスバイオサイエンス研究部, COE研究員 (60398005)
MAWATARI Kazuaki The University of Tokushima Graduate School, Institute of Health Biosciences, Assistant Professor, 大学院ヘルスバイオサイエンス研究部, 助手 (40352372)
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| Project Period (FY) |
2005 – 2006
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| Keywords | Vibrio parahaemolyticus / translation / small RNA / post transcriptional regulation / virulence factor |
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| Research Abstract |
We have examined the effect of NE on the pathogenicity of Vibrio parahaemolyticus. Norepinephrine (NE) is not only a neurotransimitter in the central nervous system, but also is known to modulate the functions of the gut, such as smooth muscle contractibility, submucosal blood flow, and active transepithelial ion transport, through interactions with α-and β-adrenergic receptors. The enterotoxicity of V. parahaemolyticus, assayed by the rat ileal loop model, was increased by NE, and the cell cytotoxicity of V.parahaemolyticus also stimulated by NE.
Furthermore, quantity of expression of mRNA of the thermostable direct haemolytsin (TDH) which was main cause of the virulence factor of V. parahemolyticus which participated in diarrhea induction did not change almost, but confirmed that production of TDH increased. And We found that Hfq was important as mRNA combination protein of TDH. This shows that production of TDH is controlled by not only mRNA expression regulation mechanism but also translation regulation mechanism from mRNA to protein.
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