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2007 Fiscal Year Final Research Report Summary

Molecular biological anslysis and clinical application of a pathological factor produced by Bartonella henselae which enhanoes human vascular endothelial cell growth

Research Project

Project/Area Number 17590396
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionKagoshima University

Principal Investigator

YOSHIIE Kiyotaka  Kagoshima University, Graduate School of Medical and Dental Sciences, Associate Professor (70174886)

Co-Investigator(Kenkyū-buntansha) ODA Hiroshi  Kagoshima University, Graduate School of Medical and Dental Sciences, Professor (40107868)
Project Period (FY) 2005 – 2007
KeywordsBartonella / Cat-scratch disease / angiogenesis / endothelial cell / neutrophil / 細胞走化性
Research Abstract

We purified a pathological factor produced by Battonella henselae which enhances human vascular endothelial cell growth. The factor was a 14kDa protein with strongly basic isoelctrical point, and seemed a kind of cellular regulatory proteins. Since this protein lost its activity after extraction from electrophoreticgel, we could not confirm the protein structure.
We also developed a new method to incubate B. henselae to promote the bacterial growth. Brucella broth was better than convenient Brain-heart infusion broth as a basic media, and B. henselae increased eataly in Brucella broth overlaid to Brucella agar supplimented with 10% rabbit blood This method improved not only isolation of bacterial products and bacterial cells but also isolation period and rate of B. hanselae from canine blood. We isolated 24 bacteria from street cats in Kagoshima, and comfirmed as B. henselae with type I gene. These isolates has similar biological activities as foloows; enhancement of vascular endothelial cell growth, induction of neutrephil chemetaxis and CCL7 expreagan, enhancement fo CCR21 secretion from lymphatic endothelial cells, and inhibition of spontaneous and induced neutrophil apoptosis. But, SDS-PAGE patterns of these isolates showed slight differences in major proteins, which suggested minor variation of naturally infected B. hensalaeto cats in Kagoshima.
We checked but could not comfirm the angiogenetic activity of conditioned medium of B. henseke in BALB/c mice in vivo. As the specificity of pathogenesis of B. henselae against animal species was unknown, further investigation was required.

  • Research Products

    (18 results)

All 2008 2007 2006 2005

All Presentation (18 results)

  • [Presentation] Bartonella henselae分離株のヒト多核白血球走化性誘導活性の比較2008

    • Author(s)
      吉家清貴, 又吉盛健, 小田紘
    • Organizer
      第81回日本細菌学会総会
    • Place of Presentation
      京都市
    • Year and Date
      2008-03-25
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Comparison of chemotactic activity of Bartonella henselae isolates to human neutrophils.2008

    • Author(s)
      Kiyotaka Yoshiie, Seiken Matayoshi, Hiroshi Oda
    • Organizer
      81^<at> Annual Meeting of Janese society for Bacteriology
    • Place of Presentation
      Kyoto
    • Year and Date
      2008-03-25
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Bartonella henselae分離株の多核白血球走化性誘導活性の比較2007

    • Author(s)
      吉家清貴, 又吉盛健, 小田紘
    • Organizer
      第77回日本感染症学会西日本地方会総会
    • Place of Presentation
      佐賀市
    • Year and Date
      2007-11-15
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Comparison of Bartonella henselae isolates in chemotactic activity to human polymorphonuclear cells.2007

    • Author(s)
      Kiyotaka Yoshiie, Seiken Matayoshi, Hiroshi Oda
    • Organizer
      77th Annual Meeting of West Japanese branch of the Japanese Association for Infectious Diseases.
    • Place of Presentation
      Saga
    • Year and Date
      2007-11-15
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Bartonella henselae迅速培養法の工夫とネコからの分離率2007

    • Author(s)
      吉家清貴, 又吉盛健, 小田紘
    • Organizer
      第80回日本細菌学会総会
    • Place of Presentation
      大阪市
    • Year and Date
      2007-03-27
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] A new method for incubation of Bartonella henselae and isolation from street cats in Kagoshima.2007

    • Author(s)
      Kiyotaka Yoshiie, Seiken Matayoshi, Hiroshi Oda
    • Organizer
      80th Annual Meeting of Janese society for Bacteriology
    • Place of Presentation
      Osaka
    • Year and Date
      2007-03-27
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Bartonella henselae迅速培養法の工夫2006

    • Author(s)
      吉家清貴, 又吉盛健, 小田紘
    • Organizer
      第76回日本感染症学会西日本地方会総会
    • Place of Presentation
      岡山市
    • Year and Date
      2006-11-23
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] A rapid and easy method for incubation of Bartonella henselae.2006

    • Author(s)
      Kiyotaka Yoshiie, Seiken Matayoshi, Hiroshi Oda
    • Organizer
      76th Annual Meeting of West Japanese branch of the Japanese Association for Infectious Diseases.
    • Place of Presentation
      Okayama
    • Year and Date
      2006-11-23
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Bartonella henselaeが有する血管内皮およびリンパ内皮細胞に対する増殖促進因子の比較2006

    • Author(s)
      吉家清貴, 前野伸昭, 又吉盛健, 小田紘
    • Organizer
      第79回日本細菌学会総会
    • Place of Presentation
      金沢市
    • Year and Date
      2006-03-29
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Comparison of enhancement activity of Bartonella henselae for human vascular and lymphatic endothelial cells.2006

    • Author(s)
      Kiyotaka Yoshiie, Nobuaki Maono, Seiken Matayoshi, Hireshi Oda
    • Organizer
      79th Annual Meeting of Janese society for Bacteriology
    • Place of Presentation
      Kanazawa
    • Year and Date
      2006-03-29
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] バルトネラ感染の細胞生物学2005

    • Author(s)
      吉家清貴
    • Organizer
      第88回日本細菌学会関東支部総会
    • Place of Presentation
      浜松市
    • Year and Date
      2005-10-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Cellular biology of Bartonella henselae.2005

    • Author(s)
      Kiyotaka Yoshiie
    • Organizer
      88th Annual Meeting of Kanto branch of the Japanese society for Bacteriology
    • Place of Presentation
      Hamamatsu
    • Year and Date
      2005-10-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Bartonella henselaeによるリンパ管内皮細胞の機能変化2005

    • Author(s)
      前野伸昭, 吉家清貴, 又吉盛健, 小田紘
    • Organizer
      第58回日本細菌学会九州支部総会
    • Place of Presentation
      那覇市
    • Year and Date
      2005-07-08
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Bartonella henselae由来好中球アポトーシス抑制因子と血管内皮細胞増殖促進因子の相互作用2005

    • Author(s)
      吉家清貴, 前野伸昭, 又吉盛健, 小田紘
    • Organizer
      第58回日本細菌学会九州支部総会
    • Place of Presentation
      那覇市
    • Year and Date
      2005-07-08
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Functional modification of human lymphatic endothelial cells by Bartonella henselae.2005

    • Author(s)
      Nobuaki Maeno, Kiyotaka Yoshiie, Seiken Matayoshi, Hiroshi Oda
    • Organizer
      58th Annual Meeting of Kyushu branch of the Japanese society for Bacteriology
    • Place of Presentation
      Naha
    • Year and Date
      2005-07-08
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Interaction of pathogenic factors of Bartonella henaelae on human vascular endothelial cell growth and neutrophil apostosis.2005

    • Author(s)
      Kiyotaka Yoshiie, Nobuaki Maeno, Seiken Matayoshi, Hiroshi Oda
    • Organizer
      58th Annual Meeting of Kyushu branch of the Japanese society for Bacteriology
    • Place of Presentation
      Naha
    • Year and Date
      2005-07-08
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 血管内皮細胞増殖促進因子のBartonella henselae培養上清からの分離精製2005

    • Author(s)
      吉家清貴, 前野伸昭, 又吉盛健, 藤村剛, 小田紘
    • Organizer
      第78回日本細菌学会総会
    • Place of Presentation
      東京都
    • Year and Date
      2005-04-06
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Purification of promoting factors on human vascular endothelial cell growth from conditioned medium of Bartonella henselae.2005

    • Author(s)
      Kiyotaka Yoshiie, Nobuaki Maeno, Seiken Matayoshi, Takeshi Fujimura, Hiroshi Oda
    • Organizer
      78th Annual Meeting of Janese society for Bacteriology
    • Place of Presentation
      Tokyo
    • Year and Date
      2005-04-06
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2010-06-09  

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