• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2006 Fiscal Year Final Research Report Summary

Virus proliferation mechanism by the monoubiquitin-dependent vesicular transport complex

Research Project

Project/Area Number 17590412
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionMiyagi Cancer Center Research Institute (2006)
Tohoku University (2005)

Principal Investigator

TANAKA Nobuyuki  Miyagi Cancer Center Research Institute, Division of Cancer Chemotherapy, Director, 免疫学部, 部長 (60280872)

Project Period (FY) 2005 – 2006
Keywordsvirus / proteome / monoubiquitin
Research Abstract

Although significance of the vesicular transport system in virus proliferation is suspected, precise mechanisms underlying it remains unknown. In this study, we investigated transport proteins STAM1, STAM2 and Hrs, all of which sort cell surface receptors, in virus life cycle. Mouse cytomegalovirus (MCMV) proliferation was decreased by the Hrs-depletion. Since MCMV attachment and entry left unaffected by Hrs, we next examined immediate early gene, ie1 mRNAs for ie1 showed no significant difference irrespective of Hrs. Unexpectedly, however, IE1 protein showed significant suppression by Hrs-depletion. I found that some portion of IE1 protein travels out of the nucleus into the cytoplasm, where it locates on the endosomes. Because Hrs is an endosomal protein, we suspect that Hrs binds and sorts Iel, leading to the lysosome dependent degradation. In conclusion, we found a novel role of a vesicular sorting protein Hrs in the regulation of a virus transcription factor, which is required for a normal virus replication.

  • Research Products

    (14 results)

All 2006 2005

All Journal Article (14 results)

  • [Journal Article] Application of HSVtk suicide gene to X-SCID gene therapy : ganciclovir treatment offsets gene corrected X-SCID B cells.2006

    • Author(s)
      Uchiyama, H., Tanaka N.ほか
    • Journal Title

      Biochem. Biophys. Res. Commun. 341

      Pages: 391-398

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Clathrin anchors deubiquitinating enzymes, AMSH and AMSH-like protein, on early endosomes.2006

    • Author(s)
      Nakamura, M., Tanaka, N.ほか
    • Journal Title

      Genes Cells 11

      Pages: 593-606

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Survival of parvovirus B19-infected cells by cellular autophagy.2006

    • Author(s)
      Nakashima A, Tanaka N. ほか
    • Journal Title

      Virology 349

      Pages: 254-263

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Murine leukemia virus vector integration favors promoter regions and regional hot spots in a human T-cell line.2006

    • Author(s)
      Tsukahara T, Tanaka, N. ほか
    • Journal Title

      Biochem Biophys Res Commun 345

      Pages: 1099-1107

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] AMSH, an ESCRT-III Associated Enzyme, Deubiquitinates Cargo on MVB/Late Endosomes.2006

    • Author(s)
      Kyuuma, M, Tanaka, N. ほか
    • Journal Title

      Cell structure & Function 31

      Pages: 159-172

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Application of HSVtk suicide gene to X-SCID gene therapy : ganciclovir treatment offsets gene corrected X-SCID B cells2006

    • Author(s)
      Uchiyama, H., Tanaka N.et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 341

      Pages: 391-398

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Clathrin anchors deubiquitinating enzymes, AMSH and AMSH-like protein, on early endosomes.2006

    • Author(s)
      Nakamura, M., Tanaka, N.et al.
    • Journal Title

      Genes Cells 11

      Pages: 593-606

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Survival of parvovirus B19-infected cells by cellular autophagy.2006

    • Author(s)
      Nakashima A, Tanaka N. et al.
    • Journal Title

      Virology 349

      Pages: 254-263

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Murine leukemia virus vector integration favors promoter regions and regional hot spots in a human T-cell line.2006

    • Author(s)
      Tsukahara T, Tanaka N. et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 345

      Pages: 1099-1107

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] AMSH, an ESCRT-III Associated Enzyme, Deubiquitinates Cargo on MVB/Late Endosomes.2006

    • Author(s)
      Kyuuma, M, Tanaka, N. et al.
    • Journal Title

      Cell structure & Function 31

      Pages: 159-172

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] TAK1, but not TAB1 or TAB2, plays an essential role in multiple signaling pathways in vivo.2005

    • Author(s)
      Shim, J., Tanaka N.ほか
    • Journal Title

      Genes. Dev. 19

      Pages: 2668-2681

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Aberrant activation of the interleukin-2 autocrine loop through the nuclear factor of activated T cells by nonleukemogenic human T-cell leukemia virus type 2 but not by leukemogenic type 1 virus.2005

    • Author(s)
      Niinuma, A., Tanaka N.ほか
    • Journal Title

      J. Virol. 79

      Pages: 11925-11934

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] TAK1, but not TAB1 or TAB2, plays an essential role in multiple signaling pathways in vivo.2005

    • Author(s)
      Shim, J., Tanaka N.et al.
    • Journal Title

      Genes.Dev. 19

      Pages: 2668-2681

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Aberrant activation of the interleukin-2 autocrine loop through the nuclear factor of activated T cells by nonleukemogenic human T-cell leukemia virus type 2 but not by leukemogenic type 1 virus.2005

    • Author(s)
      Niinuma, A., Tanaka N et al.
    • Journal Title

      J.Virol. 79

      Pages: 11925-11934

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2008-05-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi