2006 Fiscal Year Final Research Report Summary
Mechanism of maturation of herpesvirus genome DNA
| Project/Area Number |
17590420
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Fukuoka Woman's University |
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Principal Investigator |
UMENE Kenichi Fukuoka Woman's University, Faculty of human environmental science, Professor, 人間環境学部, 教授 (70127984)
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| Project Period (FY) |
2005 – 2006
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| Keywords | herpes simplex virus / HSV / herpesvirus / a sequence / genome maturation / DNA cleavage / DNA packaging / GC ratio |
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| Research Abstract |
Characteristics of herpes simplex virus type 1 (abbreviated HSV-1) are as follows:
(1) HSV-lis master herpesvirus and basic research of HSV-1 is performed at advanced level.
(2) HSV-1, which is the important human pathogene and infects 80-90% of human population, can cause severe diseases, e.g., herpes encephalitis and herpetic keratitis (the main cause of blindness).
Genome DNA maturation of HSV-1 is assumed to be done as follows:
(1) a sequence, which is the site for DNA maturation (trans-acting element), is cleaved after HSV-1 DNA replication, and virus DNA is packaged into virus particle (maturation of DNA genome).
(2) More than 10 HSV-1 genes and TAF11250 cellular gene are known to be required for DNA maturation (trans-acting elements).
<Summary of research>
The a sequence play an important role in DNA maturation of HSV-1: however, DNA sequences of the a sequence differ largely between HSV-1 isolates and it is often difficult to know effects of each element of the a sequence on the DNA m
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aturation: while these differences can be used as means for extracting essential regions of functions of the a sequence. Therefore, following experiments were performed in this study to reveal consensus sequences of the a sequence.
(1) Selection of analyzed HSV-1 strains: we have information concerning DNA polymorphisms of several hundred HSV-1 strains. Thirty HSV-1 strains were selected from these strains, considering diversity in DNA polymorphisms.
(2) Extraction of HSV-1 DNA: selected HSV-1 strains were propagated in cultured cells and HSV-1 DNA was extracted.
(3) Molecular cloning of the a sequences of HSV-1 strains: extracted HSV-1 DNA was subjected to electrophoresis after digestion with restriction endonuclease; DNA fragments containing the a sequence were recovered from the gel of electrophoresis, purified, inserted into cloning vector, and molecularly cloned.
(4) Determination of sequences of the a sequences: DNA sequences of molecularly cloned a sequences were determined.
(5) Existence of the a sequence, which have arrangements of elements and sequences differing from the a sequences reported previously, was revealed. Less
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Research Products
(10 results)
