2006 Fiscal Year Final Research Report Summary
Molecular analysis of heterogeneity in human platelet aggregation response
| Project/Area Number |
17590482
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Hirosaki University |
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Principal Investigator |
SHOJI Masaru Hirosaki University, School of Medicine, Associate Professor, 医学部, 助教授 (10226300)
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| Co-Investigator(Kenkyū-buntansha) |
YASUJIMA Minoru Hirosaki University, School of Medicine, Professor, 医学部, 教授 (90142934)
SUGIMOTO Kazuhiro Hirosaki University, University Hospital, Lecturer, 医学部附属病院, 講師 (70271799)
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| Project Period (FY) |
2005 – 2006
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| Keywords | gene expression / microRNA / vasopressin / peripheral CD34(+) stem cell / microarray / mass spectrometry |
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| Research Abstract |
There is a significant inter-individual divergence in the platelet aggregation response to arginine vasopressin (AVP). In the present study, high-throughput microarray analysis was conducted to explore molecules, which are responsible for the heterogeneity. Responders and non-responders were divided using platelet aggregation analyzer MCM hematracer 313 (Model PAM-12C). Hitachi Ace-Gene micro array analysis showed characteristic gene expression profiling. Namely, responder platelets had increased gene expressions of sodium channel, voltage-gated, type., alpha, mannose-P-dolichol utilization defect 1、eukaryotic translation termination factor 1、cysteine and glycine-rich protein 2、and general transcription factor IIH, polypeptide 2,44kDa. In non-responder platelets, genes including component of oligomeric golgi complex 6、Hermansky-Pudlak syndrome 3、PERM domain containing 1、adenosine monophosphate deaminase 2 (isoform L) nudix-type motif 1、and cell growth regulator with ring finger domain 1 were upregulated. Mass spectrometry analysis demonstrated increased platelet protein levels of protease serine 4 isoform B、and keratin 1b、trypsin family in responders and of TPMsk1、TPM3 protein、and YWHAZ protein in non-responders. There were also increased microRNA levels including hsa let 7d、hsa let 7e、hsa miR 198、hsa miR 99b、and hsa miR 98 in AVP stimulated peripheral CD34(+) stem cells (megakaryocyte progenitor cells). AVP Vla receptor gene polymorphism 242C/T showed an association with its gene expression. From these results, numbers of factors were likely to be responsible for the inter-individual heterogeneity in the platelet aggregation response to AVP.
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Research Products
(13 results)
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[Journal Article] Gene analysis of the calcium channel 1 subunit and clinical studies for two patients with hypokalemic periodic paralysis.2006
Author(s)
Kageyama K, Terui K, Tsutaya S, Matsuda E, Shoji M, Sakihara S, Nigawara T, Takayasu S, Moriyama T, Yasujima M, Suda T.
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Journal Title
J Endocrinol Invest 29
Pages: 928-33
Description
「研究成果報告書概要(欧文)」より
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