2007 Fiscal Year Final Research Report Summary
Identification and prevalence of antiseptip resistance genes in enterococci
| Project/Area Number |
17590521
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
KOBAYAHI Nobumichi Sapporo Medical University, School of Medicine Department of Hygiene, Professor (80186759)
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| Co-Investigator(Kenkyū-buntansha) |
ISHINO Masaho Sapporo Medical University, School of Medicine Department of Hygiene, Associate professor (30232325)
SUMI Ayako Sapporo Medical University, School of Medicine Department of Hygiene, Assistant professor (10363699)
NAGASHIMA Shigeo Sapporo Medical University, School of Medicine Department of Hygiene, School of Medicine Department of Hygiene (60433116)
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| Project Period (FY) |
2005 – 2007
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| Keywords | enterococcus / antiseptics / resistance gene / molecular epidemiology / multidrug efflux pump |
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| Research Abstract |
The purpose of this study is to identify antiseptic resistance genes in enterococci and clarify their epidemiological characteristics. We analyzed a total of about 1100 clinical isolates from the Sapporo Medical University Hospital obtained from 1997 to 2007, and 117 strains isolated outside this hospital. These strains were analyzed for presence of genes encoding multidrug efflux pumps, EfrA, EfrB, EmeA, and QacH, by PCR. The emeA was detected in all the E.faecalis, and efrA/efrB was found in all the isolates of E.faecalis and E.faecium. However, qacH gene was not detected in any enterococcal species. Through genetic analysis of E.faecium, at least four different kinds of genes encoding multidrug-efflux proteins were detected. Furthermore, in the whole gonome of E.faecium deposited in GenBank database, at least 80 genes encoding ABC transporters were found to be described. Enterococcal ABC transporters EfrA and EfrB are considered to belong to a same group as T LmrA of lactococcus, but different from MsrC which mediates efflux of macrolides. Further analysis on influence of sorts and numbers of drug-efflux genes on expression of antiseptic resistance in enterococci was considered to be needed in the future.
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[Journal Article] Diversity of staphylocoagulase and identification of novel variants of staphylocoagulase gene in Staphylococcus aureus2008
Author(s)
Kinoshita, M., Kobayashi, N., Nagashima, S., Ishino, M., Otokozawa, S., Mise, K., Sumi, A., Tsutsumi, H., Uehara, N., Watanabe, N., Endo, M
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Journal Title
Microbiology and Immunology (in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Analysis of the prevalence of tetracycline resistance genes in clinical isolates of Enterococcus faecalis and Enterococcus faecium in a Japanese hospital2005
Author(s)
Nishimoto, Y., Kobayashi, N., Alam, MM., Ishino, M., Uehara, N., Watanabe, N
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Journal Title
Microb Drug Resist 11
Pages: 146-153
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Detection of a novel aph(2") allele (aph(2")-Ie) conferring high-level gentamicin resistance and a spectinomycin resistance gene ant(9)-Ia (aad9) in clinical isolates of enterococci2005
Author(s)
Alam, MM, Kobayashi, N., Ishino, M., Sumi, A., Kobayashi, K., Uehara, N., Watanabe, N
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Journal Title
Microb Drug Resist 11
Pages: 239-247
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Colonizacion fecal por enterococco resistentes y virulentos en ninos ingresados en servicios pediatrico2007
Author(s)
Quinones, D., Marrero, D., Cordovez, MC, Llop, A., Kobayashi, N., del, Campo, R
Organizer
8th Central American & Caribbean Congress on Parasitology & Tropical Medicine
Place of Presentation
Havana, Cuba
Year and Date
2007-12-05
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Identification of a novel g-enotype among the low-level vancomycin resistance gene vanC-2/C-3 in Enterococcus casselnlavus2006
Author(s)
Kobayashi, N., Watanabe, S., Uehara, N., Watanabe, N., Wang, YH, Hayakawa, S., Oshima, H
Organizer
10th Western Pacific Congress on Chemotherapy and Infectious Diseases
Place of Presentation
Fukuoka, Japan
Year and Date
2006-12-05
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Genetic characterization of Panton-Valentine leukocidine-producing Staphylococcus aureus in Bangladesh2006
Author(s)
Kobayashi, N., Afroz, S., Khan, MAH, Nagashima, S., Otokozawa, S., Wang, YH
Organizer
10th Western Pacific Congress on Chemotherapy and Infectious Diseases
Place of Presentation
Fukuoka, Japan
Year and Date
2006-12-05
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Diversity of staphylocoagulase genes and identification of a putative novel coagulase type in Staphylococcus aureus2006
Author(s)
Kobayashi, N., Kinoshita, M., Tsutsumi, H., Ishino, M., Nagashima, S., Mise, K., Uehara, N., Watanabe, N
Organizer
10th Western Pacific Congress on Chemotherapy and Infectious Diseases
Place of Presentation
Fukuoka, Japan
Year and Date
2006-12-05
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Genomic diversity of low-level vancomycin resistance genes vanCl, vanC2, and vanC3 in enterococci2006
Author(s)
Kobayashi, N., Quinones, D., Ishino, M., Sumi, A., Mise, K
Organizer
The 12th International Congress on Infectious Diseases
Place of Presentation
Lisbon, Portugal.
Year and Date
2006-06-16
Description
「研究成果報告書概要(欧文)」より
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[Presentation] carriage of antibiotic-resistant enterococci in hospitalized children in Cuba2006
Author(s)
Quinones, D., Marrero, D., Cordovez, MC., Herrera, C., Kobayashi, N., Del, Campo, R., Faecal
Organizer
The 12th International Congress on Infectious Diseases
Place of Presentation
Lisbon, Portugal
Year and Date
2006-06-16
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Prevalence of Panton-Valentine leukocidin-producing Staphylococcus aureus in skin infections in Bangladesh.2006
Author(s)
Kobayashi, N., Afroz, S., Khan, AH, Ishino, M., Sumi, A., Mise, K.
Organizer
The 12th International Congress on Infectious Diseases.
Place of Presentation
Lisbon, Portugal.
Year and Date
2006-06-16
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Prevalence of aminoglycoside resistance genes in recent clinical isolates of enterococci in a Japanese hospital2005
Author(s)
Kobayashi, N., Alam, MM, Ishino, M., Sumi, A., Mise, K., Kobayashi, K
Organizer
The 24th International Congress of Chemotherapy
Place of Presentation
Manila, Philippines.
Year and Date
2005-06-05
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Prevalence of tetracycline resistance genes in clinical isolates of enterococci in a Japanese hospital2005
Author(s)
Kobayashi, N., Alam, MM, Ishino, M., Sumi, A., Mise, K
Organizer
The 24th International Congress of Chemotherapy
Place of Presentation
Manila, Philippines.
Year and Date
2005-06-05
Description
「研究成果報告書概要(欧文)」より
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