2007 Fiscal Year Final Research Report Summary
ACTION MECHANISM OF A KAMPO MEDICINE "SHOSEIRYUTO" AGAINST AIRWAY INFECTION AND ITS ACTIVE SUBSTANCE
| Project/Area Number |
17590601
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General internal medicine (including Psychosomatic medicine)
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| Research Institution | Kitasato University |
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Principal Investigator |
NAGAI Takayuji Kitasato University, KITASATO INSTITUTE FOR LIFE SCIENCES, JUNIOR ASSOCIATE PROFESSOR (00172487)
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| Co-Investigator(Kenkyū-buntansha) |
KIYOHARA Hiroaki KITASATO INSTITUTE FOR LIFE SCIENCES, 北里生命科学研究所, ASSOCIATE PROFESSOR (70161601)
SUNAZUKA Toshiaki KITASATO INSTITUTE FOR LIFE SCIENCES, 北里生命科学研究所, PROFESSOR (30226592)
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| Project Period (FY) |
2005 – 2007
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| Keywords | KAMPO MEDICINE / SHOSEIRYUTO / MUCOSAL IMMUNE SYSTEM / PROTEOMIC ANALYSIS / AIRWAY INFLAMMATION / PINELLIC ACID / INFLUENZA / ORIENTAL MEDICINE |
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| Research Abstract |
In present research, action mechanism and active ingredient of a Kampo (Japanese herbal) medicine "Shoseiryuto (SST)" against airway infection have been investigated.
1. Effect of SST against airway inflammation
Oral administration of SST reduced the airway hyperresposiveness and ovalbumin (OVA)-specific IgE antibody titer in bronchoalveolar lavage fluid (BALF) of OVA-sensitized allergic airway inflammation model mice. SST also recovered the amount of appearance of spectrin a2, a cytoskeletal protein, in lung tissue of the model mice. However prednisolone, a steroidal drug, did not reduce the OVA-specific IgE Ab titer in BALF and did not recover the amount of appearance of spectrin a2 in lung tissue of the model mice. These results indicate that action mechanism of SST is different from that of prednisolone against airway inflammation.
2. Adjuvant active substance for nasal influenza vaccine from SST
It has been appeared that SST shows a potent oral adjuvant activity for nasal influenza vaccine. We performed the isolation and characterization of an orally active adjuvant substance from the tuber of Pinellia ternata, as an active herbal component of SST. The active compound was identified as 9, 12, 13-trihydroxy-10E-octadecenoic acid (pinellic acid). However, stereoisomers of pinellic acid did not show the adjuvant activity individually, and combination of 9S, 12S, 13S and 9S, 12R, 13R stereoisomers (90.4 : 9.6) showed a potent activity. The combination with the nasal vaccine also protected the influenza virus infection. This is a first report of the combination effect of stereoisomers on the pharmacological activity of Kampo medicines.
These results suggest that SST shows its pharmacological activities through airway mucosal immune system and pinellic acid is one of active substances in SST for airway mucosal immune system.
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[Journal Article] Total synthesis, elucidation of absolute stereochemistry, and adjuvant activity of trihydroxy fatty acids.2006
Author(s)
T. Shirahata, T. Sunazuka, K. Yoshida, D. Yamamoto, Y. Harigaya, I. Kuwajima, T. Nagai, H. Kiyohara, H. Yamada, S. Omura
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Journal Title
Tetrahedron 62 (40)
Pages: 9483-9496
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Intranasally and orally effective adjuvants from Chinese and Japanese medicinal herbs for nasal influenza vaccine.2005
Author(s)
T. Nagai, H. Kiyohara, T. Sunazuka, S. Omura, H. Yamada
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Journal Title
Quality, Efficacy, Safety, Processing and Trade in Medicinal and Aromatic Plants, Acta Horticulturae, (Eds E. Brovelli et al), ISHS, Belgium No. 679
Pages: 121-129
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Application of proteomic analysis for analysis of pharmacological effects of Kampo (Japanese herbal) medicines.2008
Author(s)
T. Nagai, N. Ito, M. Nakao, R. Hashimoto, Y. Shimizu, T. Yabe, Y. Kodera, M. Oh-Ishi, T. Maeda, T. Hanawa, H. Yamada
Organizer
The 58th Symposium of the Japanese Electrophoresis Society
Place of Presentation
Tokyo (Japan)
Year and Date
2008-06-13
Description
「研究成果報告書概要(欧文)」より
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