Analysis of the new function of JAM-1 which is related to the differentiation of pancreatic endocrine cells

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 17590616
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: The University of Tokyo
• Principal Investigator: MASHIMA Hirosato The University of Tokyo, Faculty of Medicine, Research Associate (10261869)
• Project Period (FY): 2005 – 2007
• Keywords: AR42J cells / JAM-1 / tight junction (TJ) / differentiation / pancreatic endocrine cells

Research Abstract

Pancreatic AR42J cells demonstrate the pluripotency in precursor cells of the gut endoderm and also provide an excellent model system to study the differentiation of the pancreas. Using the mRNA differential display technique, we identified junctional adhesion molecule-1(JAM-1), a component of the tight junction, was highly up-regulated during the differentiation of AR42J cells, although junctions were not formed. The expression level of JAM-1 showed an up-regulation in the mRNA level after 3 hours and in the protein level after 24 hours in [activin A+betacellulin]-treated AR42J cells. The expressions of its signaling molecules, PAR-3 and atypical PKCλ, also increased after the addition of activin A+betacellulin. When JAM-1 was over-expressed in [activin A+betacellulin]-treated AR42J cells, tagged-JAM-1 was observed in cytoplasm as vesicular structures and JAM-1 was colocalized with Rab3B and Rab13, members of the Rab family expressed at tight junctions. In streptozotocin-induced regenerating islets, the expression of JAM-1 was also up-regulated in the mRNA level and the protein level. JAM-1 might therefore play an important role in the differentiation of AR42J cells and the regeneration of pancreatic islets.

Research Products

• [Journal Article] Up-regulation of JAM-1 in AR42J cells treated with activin A and betacellulin and the diabetic regenerating islets. (2008)
  • Author(s): Yoshikumi Y, Ohno H, Suzuki J, Isshiki M, Morishita Y, Ohnishi H, Yasuda H, Omata M, Fujita T, Mashima H.
  • Journal Title: Endocrine Journal (In press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Involvement of VAMP7 in Human Gastric Epithelial Cell Vacuolation Induced by Helicobacter pylori-produced VacA. (2008)
  • Author(s): Mashima H, Suzuki J, Hirayama T, Yoshikumi Y, Ohno H, Ohnishi H, Yasuda H, Fujita T, Omata M.
  • Journal Title: Infection And Immunity (In press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Roles of CTPL/Sfxn3 and Sfxn family members in pancreaticislet. (2005)
  • Author(s): Yoshikumi Y, Mashima H, Ueda N, Ohno H, Suzuki J, Tanaka S, Hayashi M, Sekine N, Ohnishi H, Yasuda H, Iiri T, Omata M, Fujita T, Kojima I.
  • Journal Title: Journal of Cellular Biochemistry 95 Pages: 1157-1168
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Roles of CTPL/Sfxn3 and Sfxn family members in pancreatic islet (2005)
  • Author(s): Yoshikumi Y, Mashima H, Ueda N, Ohno H, Suzuki J, Tanaka S, Hayashi M, Sekine N, Ohnishi H, Yasuda H, Iiri T, Omata M, Fujita T, Kojima I.
  • Journal Title: Journal of Cellular Biochemistry 95 Pages: 1157-1168
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Up-regulation of JAM-1 in AR42J cells treated with activin A and betacellulin and the diabetic regenerating islets
  • Author(s): Yoshikumi Y, Ohno H, Suzuki J, Isshiki M, Morishita Y, Ohnishi H, Yasuda H, Omata M, Fujita T, Mashima H.
  • Journal Title: Endocrine Journal (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Involvement of VAMP7 in Human Gastric Epithelial Cell Vacuolation Induced by Helicobacter pylori-produced VacA
  • Author(s): Mashima H, Suzuki J, Hirayama T, Yoshikumi Y, Ohno H, Ohnishi H, Yasuda H, Fujita T, Omata M.
  • Journal Title: Infection And Immunity (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The Role of VAMP7 on VacA-induced Vacuolation. (2007)
  • Author(s): Mashima H, Suzuki J, Yoshikumi Y, Ohno H, Omata M.
  • Organizer: Digestive Disease Week
  • Place of Presentation: Washington
  • Year and Date: 20070500
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] The Role of VAMP7 on VacA-induced Vacuolation (2007)
  • Author(s): Mashima H, Suzuki J, Yoshikumi Y, Ohno H, Omata M.
  • Organizer: Digestive Disease Week
  • Place of Presentation: Washington
  • Year and Date: 20070500
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 膵の分化におけるJAMの新たな役割 (2005)
  • Author(s): 吉汲祐加子、真嶋浩聡, 他
  • Organizer: 日本消化器病関連学会週間
  • Place of Presentation: 福岡
  • Year and Date: 20051000
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] New Role of JAM-1related to the differentiation of pancreas (2005)
  • Author(s): Yoshikumi Y, Mashima H, Suzuki J, Ohno H, Omata M.
  • Organizer: Digestive Disease Week Japan
  • Place of Presentation: Fukuoka
  • Year and Date: 20051000
  • Description: 「研究成果報告書概要(欧文)」より