2007 Fiscal Year Final Research Report Summary
Liver regeneration model using 3D hepatocyte culture system
| Project/Area Number |
17590645
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Okayama University |
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Principal Investigator |
KOIDE Norio Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Science, Professor (20142333)
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| Co-Investigator(Kenkyū-buntansha) |
SHIRAHA Hidenori Okayama University, University Hospital, Assistant Professor (40379748)
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| Project Period (FY) |
2005 – 2007
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| Keywords | 3D culture / liver regeneration / extra cellular matrix |
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| Research Abstract |
To make in vitro model of liver regeneration, we tried to introduce EGF and EGFR cDNAs into rat primary hepatocyte. Introduction of EGFR into rat primary hepatocytes increased 2 PDL. We also tried to find genes to enhance spheroid formation of hepatocyte, since hepatocyte spheroid Is known to have better differentiated function of hepatocyte compared to monolayer culture. We introduced RUNX3, a tumor suppressor gene, into hepatocellular carcinoma cell lines; Hep3B and Huh7. Introduction of RUNX3 cDNA caused conversion of cell shape in these cells. RUNX3 expression increased E-cadherin expression, while it decreased N-cadherin expression. RUNX3 cDNA introduction enhance spheroid formation that respect to better differentatid function of hepatocyte. These results suggest that sequential introduction of EGFR cDNA and RUNX3 cDNA may be a useful method for making in vitro model of liver regeneration.
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[Journal Article] Hepatitis B Virus DNA in liver Tissue and Risk for Hepatocarcinogenesis in Patients with Hepatitis C Vmts-Related Chronic Liver Disease. A Prospective Study2008
Author(s)
Obika, M., Shinji, T., Fujioka, SL., Terada, R., Ryuko, H., Lwin, AA., Shiraha, H., Koide, N
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Journal Title
Intervirology 51-1
Pages: 59-68
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Intrahepatic mRNA levels of type I interferon receptor and interferon-stimulated genes in genotype lb chronic hepatitis C. Association between IFNAR1 mRNA level and sustained response to interferon therapy2007
Author(s)
Taniguchi, H., Iwasaki, Y., Takahashi, A., Shimomura, H., Moriya, A., Yu, PC, Umeoka, F., Fujioka, S., Koide, N., Shiratori, Y
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Journal Title
Intervirology 50-1
Pages: 32-9
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Des-gamma -carboxy prothrombin-promoted vascular endothelial cell proliferation and migration2007
Author(s)
Fujikawa, T., Shiraha, H., Ueda, N., Takaoka, N., Nakanishi, Y., Matsuo, N., Tanaka, S., Nishina, SI., Suzuki, M., Takaki, A., Sakaguchi, K., Shiratori, Y
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Journal Title
J Biol Chem 282-12
Pages: 8741-8
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Xanthine oxidase-derived free radicals directly activate rat pancreatic stellate cells2006
Author(s)
Tanioka, H., Mizushima, T., Shirahige, A., Matsushita, K., Ochi, K., Ichimura, M., Matsumura, N., Shinji, T., Tanimoto, M., Koide, N
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Journal Title
J Gastroenterol Hepatol 21-3
Pages: 537-44
Description
「研究成果報告書概要(欧文)」より
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