過敏性腸症候群の原因としてのCHRM3およびβ3AR遺伝子多型の研究

2005 Fiscal Year Annual Research Report

• Project/Area Number: 17590671
• Research Category: Grant-in-Aid for Scientific Research (C)
• Research Institution: Iwate Medical University
• Principal Investigator: 千葉 俊美 岩手医科大学, 医学部, 講師 (40254784)
• Co-Investigator(Kenkyū-buntansha): 折居 正之 岩手医科大学, 医学部, 助教授 (00158833) ; 菅井 有 岩手医科大学, 医学部, 助教授 (20187628)
• Keywords: 過敏性腸症候群 / CHRM3 / β3AR / 遺伝子多型

Research Abstract

消化管機能疾患外来にて患者診察を行いながら、RomeII基準を用いて診断得られた過敏性腸症候群(Irritable Bowel Syndrome ; IBS)患者を厳選し、既に、本学の倫理委員会より遺伝子検索に基づく承認を得て、患者からの同意書を作成しており、それに基づいてIBS患者に、本研究の目的、方法および検体の匿名化などを説明た後、同意を得られた患者より血液検体を採取し、末梢血液中のゲノムDNAを抽出している。検体は匿名化しプライバシーの秘守に全面的に配慮して実施している。また、PCR増幅装置としてGold96-Well GeneAmp PCRシステム9700を設置し、現在CHRM3およびβ_3ARプライマーの選択に入っているところである。今後は、現在収集している検体をもとにCholinergic receptor muscarinic 3(CHRM3)およびβ3-adrenoceptor(β_3AR)遺伝子polymorphismの検出を試みる。また、健常人としてのコントロール群の検体も同様に同意を得て収集し検索予定である。さらに、平成17年度は、過敏性腸症候群を含む機能性機能異常症や遺伝子検索に基づく論文発表を行いながら、本研究の遂行中である。

Research Products

• [Journal Article] Effects of itopride hydrochloride and ranitidine in patients with functional dyspepsia : comparison between prokinetic and acid suppression therapies. (2006)
  • Author(s): Chiba T, Tokunaga Y, Ikeda K, Takagi R, Chishima R, Terui T, M, Kudara N, Endo M, Inomata M, Orii S, Suzuki K.
  • Journal Title: Hepatogastroenterology (In press)
• [Journal Article] Analysis of molecular alterations in left- and right-sided colorectal carcinomas reveals distinct pathways of carcinogenesis : proposal for new molecular profile of colorectal carcinomas. (2006)
  • Author(s): Sugai T, Habano W, Jiao Y-F, Tsukahara M, Takeda Y, Otsuka K, Nakamura S.
  • Journal Title: J Mol Diagn (in press)
• [Journal Article] Clinicopathologic significance of loss of heterozygosity in intestinal and solid type gastric carcinomas : a comprehensive study using the crypt isolation technique. (2006)
  • Author(s): Jiao Y-F, Sugai T, Habano W, Uesugi N, Takagane T, Nakamura S.
  • Journal Title: Mod Pathol (in press)
• [Journal Article] Colonic transit, bowel movements, stool form, and abdominal pain in irritable bowel syndrome by treatments with calcium polycarbophil. (2005)
  • Author(s): Chiba T, Kudara N, Sato M, Chishima R, Abiko Y, Inomata M, Orii S, Suzuki K.
  • Journal Title: Hepatogastroenterology 52・65 Pages: 1416-1420
• [Journal Article] Upper gastrointestinal disorders induced by non-steroidal anti-inflammatory drugs. (2005)
  • Author(s): Chiba T, Sato K, Endo M, Ando T, Inomata M, Orii S, Suzuki K.
  • Journal Title: Hepatogastroenterology 52・64 Pages: 1134-1138
• [Journal Article] Influence of Gelatin-Resorcin-Formalin Glue on mild-term redissection after aortic repair. (2005)
  • Author(s): Nakajima T, Kawazoe K, Izumoto H, Kawada T, Kataoka T, Yoshioka K, Sugai T.
  • Journal Title: Surg Today 35 Pages: 112-116
• [Journal Article] Analysis of allelic imbalances at multiple cancer-related chromosomal loci and microsattellite instability within the same tumor using a single tumor gland from colorectal carcinomas. (2005)
  • Author(s): Sugai T, Habano W, Jiao Y-F, Suzuki M, Takagi R, Otsuka K, Higuchi T, Nakamura S.
  • Journal Title: Int J Cancer 114 Pages: 337-345
• [Journal Article] Analysis of genetic alterations associated with DNA diploidy, aneuploidy and multiploidy in gastric cancers. (2005)
  • Author(s): Sugai T, Habano W, Jiao Y-F, Suzuki M, Takagane A, Nakamura S.
  • Journal Title: Oncology 68 Pages: 548-557
• [Journal Article] Analysis of SMAD4/DPC4 gene alterations in multiploid colorectal carcinomas. (2005)
  • Author(s): Ando T, Sugai T, Habano W, Jiao Y-F, Suzuki K.
  • Journal Title: J gastroenterol 40 Pages: 708-715
• [Journal Article] Mapping of the methylation pattern of the MUC2 promoter in pancreatic cancer cell lines, using bisulfite genomic sequencing. (2005)
  • Author(s): Hamada T, Goto M, Tsutsumida H, Nomoto M, Higashi M, Sugai T, Nakamura S, Yonezawa S.
  • Journal Title: Cancer Let 227 Pages: 175-184
• [Journal Article] Clinicopathological study of depth of subserosal invasion in patients with pT2 gallbladder carcinoma. (2005)
  • Author(s): Sasaki R, Uesugi N, Itabashi H, Fujita T, Takeda Y, Hoshikawa K, Takahashi M, Funato O, Nitta H, Sugai T, Kanno S, Saito K.
  • Journal Title: J Surg Oncol 92 Pages: 83-88
• [Journal Article] Late complications following surgery for type a acute aortic dissection using gelatin-resorcin-formaldehyde glue : report of two cases. (2005)
  • Author(s): Kamada T, Nakajima T, Izumoto H, Sugai T, Yoshioka K, Kawazoe K.
  • Journal Title: Surg Today 35 Pages: 996-999