2006 Fiscal Year Final Research Report Summary
Research for biochemical diagnosis using a-synuclein level in the CSF of patients with neurodegenerative disease
| Project/Area Number |
17590869
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Shinshu Universuty |
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Principal Investigator |
IKEDA Shu-ichi Shinshu Universuty, School of Medicine, Professor, 医学部, 教授 (60135134)
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| Co-Investigator(Kenkyū-buntansha) |
KAKEI Yoichi Shinshu Universuty, School of Medicine, Lecturer, 医学部, 講師 (90273086)
KANEKO Kazuma Shinshu Universuty, School of Medicine, assistant, 医学部, 助手 (80402105)
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| Project Period (FY) |
2005 – 2006
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| Keywords | Parkinson's disease / Multiple system atrophy / α-synuclein / ELISA / Dementia with Lewy body |
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| Research Abstract |
We have established an ELISA system for precise α-synuclein concentration in human biological fluid, especially cerebrospinal fluid (CSF). To evaluate the intracorporeal metabolism ofα-synuclein, we measured this protein levels by the ELISA system both in plasma and CSF. There were no relation between a-synuclein level in the plasma and that in the CSF. Therefore we decided to investigate only CSF to be considered reflecting clinical condition. The CSF samples of the patients with three types of synucleinopathy, such as Parkinson's disease, diffuse Lewy body disease and multiple system atrophy, were examined. The α-synuclein concentration in the CSF with synucleinopathy was significantly lower than that with other neurological disease with parkinsonism. There was no significant differences between three synucleinopathies. To distinguish Parkinson's disease from multiple system atrophy, we established combined diagnostic system using α-synclein concentration in CSF and [123I]MIBG cardiac scintigraophy. Significant decrease of [123I]MIBG cardiac uptake with Parkinson's disease patient, whilst normal uptake with multiple system atrophy patients were shown same as previous reports. Therefore, it has been possible to separate PD and multiple system atrophy from other neurodegenerative disease with parkinsonism by the use of a-synuclein level at first. Then PD has been distinguished from multiple system atrophy by the use of [123I]MIBG cardiac scintigraphy.
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