2006 Fiscal Year Final Research Report Summary

Research for cell therapy using NK cell receptor-expressing T cells against leukemia.

Research Project

Project/Area Number	17590975
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Hematology
Research Institution	Hokkaido University
Principal Investigator	TANAKA Junji Hokkaido University, Graduate School of Medicine, Associate Professor, 大学院医学研究科, 助教授 (50250452)
Project Period (FY)	2005 – 2006
Keywords	Cell therapy / NK cell / GVHD / GVL / Stem cell transplantation / Leukemia
Research Abstract	In this study, we could expand inhibitory NK cell receptor (CD94/NKG2A)-expressing CD8 T cells from G-CSF-mobilized peripheral blood mononuclear cells (G-PBMC), patients' derived autologous G-CSF-mobilized peripheral blood mononuclear cells (Auto G-PBMC) and cord blood by the stimulation of IL-15 and OKT3. These expanded CD94 /NKG2A-expres sing CD8 T cells could attack HLA class I-low leukemic cell line such as K562 and patients' derived primary leukemic cells. Also, it was reveled that activating NK cell receptor NKG2D and it's legand MICA/B were involved in the killing activity of CD94/NKG2A-expressing CD8 T cells.

(3 results)