2006 Fiscal Year Final Research Report Summary
The mechanism of translocation of CD45R0 to lipid rafts in IL-6-induced proliferation of human myeloma cells
| Project/Area Number |
17591000
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
KAWANO Michio Yamaguchi University, Graduate School of Medicine, Professor, 大学院医学系研究科, 教授 (40161343)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIKAWA Hideaki Yamaguchi University, Graduate School of Medicine, Associate Professor, 大学院医学系研究科, 助教授 (40294623)
OBATA Masanori Yamaguchi University, Graduate School of Medicine, Assistant Professor, 大学院医学系研究科, 講師 (80158831)
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| Project Period (FY) |
2005 – 2006
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| Keywords | myeloma cells / CD45 / CD45R0 / CD45RA / lipid rafts / signal transduction / Galectin-1 / IL-6 |
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| Research Abstract |
Human myeloma cells show the different surface antigen expressions from those in norma bone marrow plasma cells; especially, all normal plasma cells express CD45, CD45RA, but most myeloma cells do not express CD45 and a small subpopulation of myeloma cells express CD45, CD45R0 but not CD45RA. Myeloma cells respond to interleukin 6(IL-6) to proliferate, and this IL-6-induced proliferation of myeloma cells requires CD45, CD45R0 on their surface. In order to clarify the role of CD45 (CD45R0) on the IL-6-induced proliferation, we investigated how CD45R0 or CD45RA translocated to lipid rafts after IL-6 stimulation in human myeloma cell lines. CD45R0 rapidly translocated to lipid rafts after IL-6 stimulation, while CD45RA remained outside of lipd rafts. The presence of CD45RA induced the suppression of intracellular signaling induced by IL-6, especially the downregulation of phosphorylation of ERK. Also, we investigated what molecules could bind to CD45RA but not CD45R0 extracellularly ; Galectin-1 could bind to CD45RA but not CD45RO, and induced the suppression of IL-6-induced proliferation. Therefore, these results suggest that IL-6-induced proliferation of myeloma cells is regulated by CD45RO, and CD45RA has the inhibitory effect on IL-6-induced proliferation and the mechanism of the inhibitory effect of CD45RA may be due to the blockade of translocation to lipid rafts and the extracellualr binding of Galectin-1.
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[Book] 三輪血液病学2006
Author(s)
河野道生
Total Pages
2070
Publisher
文光堂
Description
「研究成果報告書概要(和文)」より
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