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2006 Fiscal Year Final Research Report Summary

The mechanism of translocation of CD45R0 to lipid rafts in IL-6-induced proliferation of human myeloma cells

Research Project

Project/Area Number 17591000
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionYamaguchi University

Principal Investigator

KAWANO Michio  Yamaguchi University, Graduate School of Medicine, Professor, 大学院医学系研究科, 教授 (40161343)

Co-Investigator(Kenkyū-buntansha) ISHIKAWA Hideaki  Yamaguchi University, Graduate School of Medicine, Associate Professor, 大学院医学系研究科, 助教授 (40294623)
OBATA Masanori  Yamaguchi University, Graduate School of Medicine, Assistant Professor, 大学院医学系研究科, 講師 (80158831)
Project Period (FY) 2005 – 2006
Keywordsmyeloma cells / CD45 / CD45R0 / CD45RA / lipid rafts / signal transduction / Galectin-1 / IL-6
Research Abstract

Human myeloma cells show the different surface antigen expressions from those in norma bone marrow plasma cells; especially, all normal plasma cells express CD45, CD45RA, but most myeloma cells do not express CD45 and a small subpopulation of myeloma cells express CD45, CD45R0 but not CD45RA. Myeloma cells respond to interleukin 6(IL-6) to proliferate, and this IL-6-induced proliferation of myeloma cells requires CD45, CD45R0 on their surface. In order to clarify the role of CD45 (CD45R0) on the IL-6-induced proliferation, we investigated how CD45R0 or CD45RA translocated to lipid rafts after IL-6 stimulation in human myeloma cell lines. CD45R0 rapidly translocated to lipid rafts after IL-6 stimulation, while CD45RA remained outside of lipd rafts. The presence of CD45RA induced the suppression of intracellular signaling induced by IL-6, especially the downregulation of phosphorylation of ERK. Also, we investigated what molecules could bind to CD45RA but not CD45R0 extracellularly ; Galectin-1 could bind to CD45RA but not CD45RO, and induced the suppression of IL-6-induced proliferation. Therefore, these results suggest that IL-6-induced proliferation of myeloma cells is regulated by CD45RO, and CD45RA has the inhibitory effect on IL-6-induced proliferation and the mechanism of the inhibitory effect of CD45RA may be due to the blockade of translocation to lipid rafts and the extracellualr binding of Galectin-1.

  • Research Products

    (10 results)

All 2007 2006 2005

All Journal Article (8 results) Book (2 results)

  • [Journal Article] PPAR β-mediated growth suppression of baicalein and dexamethasone in human myeloma cells.2007

    • Author(s)
      Otsuyama K
    • Journal Title

      Leukemia 21

      Pages: 187-190

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] PPARβ-mediated growth suppression of baicalein and dexamethasone in human myeloma cells.2007

    • Author(s)
      Otsuyama K
    • Journal Title

      Leukemia 21

      Pages: 187-190

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Increased susceptibility to apoptosis in CD45(+) myeloma cells accompanied by the increased expression of VDAC-1.2006

    • Author(s)
      Liu S
    • Journal Title

      Oncogene 25

      Pages: 419-429

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] An increase in MPC-1- and MPC-1-CD45+ immature Myeloma cells in the progressive states of bone marrow plasmacytosis.2006

    • Author(s)
      Otsuyama K
    • Journal Title

      Int J Hematol 83

      Pages: 39-43

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Heterogeneous expression of CD32 and CD32-mediated growth suppression in human myeloma cells.2006

    • Author(s)
      Zheng X
    • Journal Title

      Haematologica 91

      Pages: 920-928

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] An increased in MPC-1-and MPC-1-CD45+ immature myeloma cells in the progressive states of bone marrow plasmacytosis.2006

    • Author(s)
      Otsuyama K
    • Journal Title

      Int J Hematol 83

      Pages: 39-43

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma.2005

    • Author(s)
      Li F
    • Journal Title

      Blood 105

      Pages: 3295-3302

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A rapid translocation of CD45R0 but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma.2005

    • Author(s)
      Li F
    • Journal Title

      Blood 105

      Pages: 3295-3302

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 三輪血液病学2006

    • Author(s)
      河野道生
    • Total Pages
      2070
    • Publisher
      文光堂
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 多発性骨髄腫 Up-to-date2006

    • Author(s)
      河野道生
    • Total Pages
      191
    • Publisher
      メディカルレビュー社
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2008-05-27  

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