2007 Fiscal Year Final Research Report Summary
Establishment of stratification of therapeutic methods by new prognostic factor nm23 in patients with non-Hodgkin's lymphomas
| Project/Area Number |
17591008
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Saitama Medical University |
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Principal Investigator |
NIITSU Nozomi Saitama Medical University, Faculty of Medicine, Professor (20256697)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Non-Hodgkin lymphoma / nm23-H1 / Rituximab / ELISA / RT-PCR |
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| Research Abstract |
We previously detected nm23-H1 protein in the serum of patients with lymphoma, and developed a method of measuring the serum nm23-H1 level by ELISA. In patients with non-Hodgkin's lymphomas, we reported that a high serum nm23-H1 level was a poor prognostic factor. Since there are still many unanswered questions about the role of serum nm23-Hl protein in lymphomas, we studied nm23-H1 protein expression in lymphomas and its localization in cells by immunohistochemistry and performed functional analysis of nm23-H1 in lymphomas. (1) As one of the functional analyses of nm23-H1, we examined nm23 mRNA expression in lymphoma cells. We extracted RNA from lymph nodes and from lymphoid tissues of lymphomas and examined nm23 expression quantitatively by RT-PCR. We found that the level of mRNA expression of nm23-H1 was associated with the" or "was correlated with the serum nm23-H1 level and found that it was a prognostic factor of lymphoma similar to serum nm23-H1 (2) High serum nm23-H1 was a poor
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prognostic factor for not only untreated non-Hodgkin lymphoma cases but also relapsed cases, and a prognostic factor in patients with lymphoma that was refractory to chemotherapy. In addition, we reported that high serum nm23-H1 was associated with early relapse. (3) In patients with Hodgkin's lymphoma, we examined serum and intracytoplasmic nm23, and intracytoplasmic nm23 was found in a large percentage of Hodgkin's cells. In addition, the levels of serum and intracytoplasmic nm23-H1 were independent prognostic factors in patients with Hodgkin's lymphoma similar to patients with non-Hodgkin's lymphomas. (4) We examined whether high serum nm23-H1 was a prognostic factor for diffuse large B-cell lymphoma. In diffuse large B-cell lymphoma that was treated with rituximab combination chemotherapy, we found that nm23-H1 became an independent prognostic factor. (5) In primary thyroid lymphomas and primary breast lymphomas, we found that nm23-H1 overexpression was a significant poor prognostic factor. Less
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