2006 Fiscal Year Final Research Report Summary
Functional analysis of cell adhesion molecule, JAM-4S, specifically expressed in tissue specific stem cells.
| Project/Area Number |
17591009
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Yokohama City University (2006)
Keio University (2005) |
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Principal Investigator |
OHBO Kazuyuki Yokohama City University, School of Medicine, Associate Professor, 医学部, 準教授 (70250751)
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| Project Period (FY) |
2005 – 2006
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| Keywords | Cell and Tissue / Regeneration Medicine / Development and Differentiation / Biological Molecule |
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| Research Abstract |
In this project, we focused on the analysis of molecule(s), especially cell surface molecule(s), which are commonly expressed in several tissue specific stem cells because tissue specific stem cells show quite similar properties. Then, we have cloned one of the cell adhesion molecules, JAM-4, which are expressed in both hematopoietic stem cells and male germ stem cells.
The JAM-4 transcript, named JAM-4S, which we have cloned from stem cells is shorter than that cloned from kidney and lung. The transcript encoded an isoform of JAM-4 protein, which only contain one immunoglobulin domain. Therefore, we analyzed the function of JAM-4S protein as well as JAM-4 protein in the stem cells at male reproduction system and hematopoietic system. In testes, JAM-4S is expressed in gonocytes and spermatogonia at neonatal stage. In hematopoietic system, JAM-4S is expressed in both stem cell-and progenitor-populations in bone marrow cells.
In order to understand the biological function in vivo, we have generated the JAM-4 deficient mice. Because it is difficult to generate JAM-4S specific knock out mice, we have generated conventional JAM-4 knock out mice. The JAM-4 deficient mice alive at least 2 years so far and do not show obvious abnormality. The testes specimen of JAM-4 deficient mice showed normal development. The hematopoiesis of the JAM-4 deficient mice was also normal. In testes and bone marrow, the family molecules of JAM-4, such as JAM-A and JAM-B are also expressed. Therefore, we are going to generate JAM-B/JAM-4-double knock out mice to analyze the phenotype on stem cell population.
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[Journal Article] Loss of Tie2 receptor compromises embryonic stem cell-derived endothelial but not hematopoietic cell survival.2006
Author(s)
Hamaguchi, I., Morisada, T., Azuma, M., Murakami, K., Kuramitsu, M., Mizukami, T., Ohbo, K., Yamaguchi, K., Oike, Y., Dumont, DJ., Suda, T.
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Journal Title
Blood 107
Pages: 1207-1213
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The first round of mouse spermatogenesis lacks the stem cell stage2006
Author(s)
Yoshida, S., Sukeno, M., Nakagawa, T., Ohbo, K., Nagamatsu, G., Suda, T., Nabeshima, Y-I.
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Journal Title
Development 133
Pages: 1495-1505
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A CTX family cell adhesion molecule, JAM4, is expressed in stem cell and progenitor cell populations of both male germ cell and hematopoietic cell lineages.2006
Author(s)
Nagamatsu, G, Ohmura, M., Mizukami, T., Hamaguchi, I., Hirabayashi, S., Yoshida, S., Hata, Y., Suda, T., Ohbo, K.
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Journal Title
Mol Cell Biol. 26
Pages: 8498-8506
Description
「研究成果報告書概要(欧文)」より
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