2007 Fiscal Year Final Research Report Summary
Investigation of CD45-negative hematopoietic cells
| Project/Area Number |
17591015
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
OGATA KiYoYuki Nippon Medical School, Department of Medicine, Professor (20169171)
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| Co-Investigator(Kenkyū-buntansha) |
TAMURA Hideto Nippon Medical School, Department of Medicine, Assistant Professor (70256949)
TSUJI Takashi Tokyo University of Science, Department of Industrial Science, Professor (50339131)
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| Project Period (FY) |
2005 – 2007
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| Keywords | myelodysplastic syndromes / Leukemic stem cells / CD45 / CD7 |
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| Research Abstract |
We identified that blasts lacking CD45 molecules exist in the bone marrow from patients with myelodysplastic syndromes (MDS). We could not document the stem cell activity in these CD45-negative cells. However, because MDS stem cells are very unstable, we could not deny the possibility that these cells are MDS stem cells. During the above study, we found that a small fraction of CD45-negative cells express CD7 molecules. Then, we compared the cell characteristics between CD7-positive and CD7-negative cells. CD7-positive cells are more proliferative and less prone to apoptosis compared with CD7-negative cells.
The thinking process in the above study relating to MDS progenitor/stem cells has made us hypothesize a new theory of leukemic stem cells. That is, cells, which have self renewal potential, and therefore not simple progenitors, and their differential capability is limited to the myeloid lineage, exist in humans. These cells (myeloid stem cells) may be transformed in MDS and other myeloid malignancies.
Furthermore, in the analyses of immature blasts in MDS, we found that immunophenotype is often deranged in MDS blasts, which is usable for making/confirming the diagnosis of MDS.
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[Journal Article] Flow cytometric parameters with little interexaminer variability for diagnosing low-grade myelodysplastic syndromes2008
Author(s)
Satoh, C, Dan, K, Yamashita, T, Jo, R, Tamura, H, Ogata, K
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Journal Title
Leukemia Research 32-5
Pages: 699-707
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification and hematopoietic potential of CD45- clonal cells with very immature phenotype (CD45-CD34-CD38-Lin-) in patients with myelodysplastic syndromes2005
Author(s)
Ogata, K, Satoh, C, Tachibana, M, Hyodo, H, Tamura, H, et. al.
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Journal Title
Stem Cells 23-5
Pages: 619-630
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Biological significance of CD7-positive blasts in myelodysplastic syndromes2007
Author(s)
Satoh, C, Tamura, H, Yamshita, T, Tsuji, T, Dan, K, Ogata, K
Organizer
The 9th International MDS symposium
Place of Presentation
Florence, Italy
Year and Date
20070500
Description
「研究成果報告書概要(欧文)」より
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