2006 Fiscal Year Final Research Report Summary
Expression and functional analysis of cellular anti-HIV factor among HIV-infected Long-Term Nonprogressor
| Project/Area Number |
17591062
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | Osaka National Hospital, National Health Organization |
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Principal Investigator |
YAMAMOTO Yoshihiko Osaka National Hospital, National Health Organization, Investigator, 研究員 (30393292)
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| Co-Investigator(Kenkyū-buntansha) |
SHIRASAKA Takuma Osaka National Hospital, National Health Organization, 部長 (40359310)
UCHIYAMA Tsuneo Graduate School of Tokushima University, Associate Professor, 大学院, 助教授 (90151901)
GATANAGA Hiroyuki International Medical Center of Japan, AIDS Clinical Center, 厚生労働技官
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| Project Period (FY) |
2005 – 2006
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| Keywords | HIV / Long Term Nonprogressors (LTNP) / AOBEC3G / vif |
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| Research Abstract |
1,Search of HIV-infected Long Term Nonprogressors
We searched the patients who maintained CD4+-T Cell count more than 500 for recent six years among more than 2100 patients who hospitalized to Osaka National hospital and International Medical Center of Japan. As a result, 15 patients were found.
2,Expression of APOBEC3G
We received anti-APOBEC3G antibody and it successfully showed the high expression of intracellular APOBEC3G through the FI assay. However, this expression level in PBMC goes up and down in each lot. Thus, we constructed the standard cell line which expresses the APOBEC3G protein constantly, and this cell line was useful for the normalization of APOBEC3G. Generally, non-Adherent cells were used for the HIV infection assay. However, it is difficult to make and maintain the appropriate APOBEC3G expression constantly. Then, we establish the cell line which is based on the adherent cell line, 293T cells. Among the clone A3G-C1 and A3G-C4, vif-defected HIV could not infect with. APOBEC3G expression in these cells was high and functional. By these cell line, quantification of APOBEC3G is possible and the measurement of APOBEC3G expression among Long Term Nonprogressors and slow progressors.
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Research Products
(33 results)
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[Journal Article] Drug-resistant HIV-1 prevalence in patients newly diagnosed with HIV/AIDS in Japan.2007
Author(s)
Gatanaga H, Ibe S, Matsuda M, Yoshida S, Asagi T, Kondo M, Sadamasu K, Tsukada H, Masakane A, Moti H, Takata N, Minami R, Tateyama M, Koike T, Itoh T, Imai M, Nagashima M, Gejyo F, Ueda M, Hamaguchi M, Kojima Y, Shirasaka T, Kimura A, Yamamoto M, Fujita J, Oka S, Sugiura W.
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Journal Title
Antiviral Research (in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Urinary 2-microglobulin as a possible sensitive marker for renal injury caused by tenofovir clisproxil fumarate.2006
Author(s)
Gatanaga H, Tachikawa N, Kikuchi Y, Teruya K, Genka I, Honda M, Tanuma J, Yazaki H, Ueda A, Kimura S, Oka S.
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Journal Title
SIDS Research and Human Retroviruses Vol.22
Pages: 744-748
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Structure-based design of novel HIV-1 protease inhibitors to combat drug resistance.2006
Author(s)
Ghosh AK, Sridhar PR, Leshchenko S, Hussain AK, Li J, Kovalevsky AY, Walters DE, Wedekind JE, Grum-Tokars V, Das D, Koh Y, Maeda K, Gatanaga H, Weber IT, Mitsuya H.
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Journal Title
Journal of Medical Chemistry Vol.49
Pages: 5252-5261
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Distinct expression of Kaposi's sarcoma-associated herpesvirus-encoded proteins in Kaposi's sarcoma and multicentric Castleman's disease.2006
Author(s)
Abe Y, Matsubara D, Gatanaga H, Oka S, Kimura S, Sasao Y, Saitoh K, Fujii T, Sato Y, Sata T, Katano H.
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Journal Title
Pathology International Vol.56
Pages: 617-624
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] 2007. A protein from a parasitic microorganism, Rickettsia prowazekii, can cleave the signal sequences of proteins targeting mitochondria.
Author(s)
Kitada, S., Uchiyama, T., Funatsu, T., Kitada, Y., Ogishima, T., Ito, A.
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Journal Title
Journal of Bacteriology 189
Pages: 844-850
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] 2006. Murine typhus from Vietnam, imported into Japan.
Author(s)
Azuma, M., Nishioka, Y., Ogawa, M., Takasaki, T., Sone, S., Uchiyama, T.
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Journal Title
Emerging Infectious Diseases. 12
Pages: 1466-1468
Description
「研究成果報告書概要(欧文)」より
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