2007 Fiscal Year Final Research Report Summary
Biofilm formation and related pathogenic genes of clinical isolates from children with prolonged infections
| Project/Area Number |
17591098
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Kagoshima University |
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Principal Investigator |
NISHI Junichirou Kagoshima University, Medical and Dental Hospital, Senior Assistant Professor (40295241)
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| Co-Investigator(Kenkyū-buntansha) |
NOMURA Yuichi Kagoshima University, Graduate School of Medical and Dental Sciences, Associate Professor (90237884)
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| Project Period (FY) |
2005 – 2007
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| Keywords | prolonged infection / biofilm / Enteroaggregative E. coli / AatA / TolC / H. influenzae / MRSA / agr |
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| Research Abstract |
Biofilm formation and related pathogenic genes of clinical isolates from children with prolonged infections were investigated. Enteroaggregative Escherichia coli (EAEC) is an emerging enteric pathogen in both developing and industrialized countries. AatA, an outer-membrane protein that is a homolog of E. call To1C, facilitates the export of the dispersin protein Aap. The structure-function analysis of AatA demonstrated that three nonpolar amino acids at positions 381-383 (Phe-Leu-Leu) were required for the activity. We investigated the role of To1C in the virulence of EAEC, and showed that To1C promotes aggregation and adhesion of EAEC by secreting an assumed humoral factor. In addition, we demonstrated that the shf gene on the pathogenic plasmid is required for the firm biofilm formation of EAEC. Adhesins such as HifA, HMW1/2, Ilia, Hap of nontypable Haemophilus influenzae were investigated in clinical isolates from children. We concluded that HifA and'Hia are associated with bioflm formation, and that biofilm formation plays an important role in recurrent respiratory infections. Biofilm formation and the accessory gene regulator (agr) typing of MRSA strains were investigated. The biofilm index and the incidence of agr-2 in the infection group were significantly higher than those in the carrier group, suggesting that biofilm formation and agr type are associated with nosocomial MRSA infections. These results are considered to be significant for further studies to develop the new therapy for prolonged infections associated with biofilm in children.
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[Journal Article] Escherichia coli efflux pump TolC promotes aggregation of enteroaggregative E. coli 0422008
Author(s)
Imuta, N., Nishi, J., Tokuda, K., Fujiyama, R., Manago, K., Iwashita, M., Sarantuya, J., Kawano, Y
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Journal Title
Infect Immun 76(3)
Pages: 1247-1256
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The shf gene of a Shigella flexneri homolog on the virulent plasmid pAA2 of enteroaggregative Escherichia coli 042 is required for firm biofilm formation2008
Author(s)
Fujiyama, R., Nishi, J., Imuta, N., Tokuda, K., Manago, K., Kawano, Y
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Journal Title
Current Microb 56(5)
Pages: 474-80
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Proinflammatory cytokines in cerebrospinal fluid from patients with nontyphoidal Salmonella encephalopathy2008
Author(s)
Imamura, M., Nishi, J., Tamada, I., Tenokuchi, Y., Toyoshima, M., Kawano, Y
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Journal Title
Pediatr Infect Dis J 27 (in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A childhood case of descending necrotizing mediastinitis secondary to retropharyngeal abscess2008
Author(s)
Yamasaki, Y., Nishi, J., Nishikawa, T., Tatsumoto, C., Kawano, F., Sano, N., Kawano, Y., Kawakami, K
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Journal Title
J Infect Chemother (in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Multifocal osteomyelitis due to Bartonella henselae in a child without focal nain2007
Author(s)
Kodama, Y., Maeno, N., Nishi, J., Imuta, N., Oda, H., Tanaka, S., Kono, Y., Kawano, Y
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Journal Title
J Infect Chemother 13(5)
Pages: 350-352
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Biofilm formation by and accessory gene regulator typing of methicillin-resistant Staphylococcus aureus strains recovered from patients with nosocomial infections.2006
Author(s)
Manago, K., Nishi, J., Wakimoto, N., Miyanohara, H., Sarantuya, J., Tokuda, K., Iwashita, M., Yamamoto, K., Yoshinaga, M., Maruyama, I., Kawano, Y
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Journal Title
Infect Control Hosp Epidemiol 27(2)
Pages: 188-90
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Role of the carboxy-terminal region of the outer membrane protein AatA in the export of dispersin from enteroaeereeative Escherichia coli.2006
Author(s)
Iwashita, M., Nishi, J., Wakimoto, N., Fujiyama, R., Yamamoto, K., Tokuda, K., Manago, K., Kawano, Y
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Journal Title
FEMS microbiol lett 256(2)
Pages: 266-72
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Immune response to a laminin-binding protein (Lmb) in group A streptococcal infection2005
Author(s)
Wahid, MR., Yoshinaga, M., Nishi, J., Maeno, N., Sarantuya, J., Ohkawa, T., Jalil, AM, Kobayash, K., Miyata, K
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Journal Title
Pediatr Int. 47(2)
Pages: 196-202
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A neonatal case of adrenal and cephalhematoma abscesses caused by Escherichia coli after forceps delivery2005
Author(s)
Nishi, J., Kaji, T., Tokuda, K., Shinkoda, Y., OkaWa, T., Noguchi, H., Takamatsu, H., Yoshinaga, M
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Journal Title
Pediatr Int. 47(4)
Pages: 480-2
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Incidence and O-serotypes of diarrheagenic Escherichia coli among E. coli isolated from diarrheal children in Japan -EAEC and atypical EPEC as unrecognized enteric pathogens-2007
Author(s)
Nishi, J, Imuta, N., Fujiyama, R., Manago, K., Kamenosono, A., Tokuda, K
Organizer
107th General Meeting of American Society for Microbiology
Place of Presentation
Toronto, Canada
Year and Date
2007-05-24
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Heterogeneity of acid-resistance among Enteroaggregative Escherichia coli (EAEC) clinical isolates : typical EAEC is more highly acid-resistant than atypical EAEC.2005
Author(s)
Wakimoto, N., Nishi, J., Fujiyama, R., Tokuda, K., Yoshinaga, M., Kawano, Y
Organizer
105th General Meeting of American Society for Microbiology
Place of Presentation
Atlanta, USA
Year and Date
20050606-07
Description
「研究成果報告書概要(欧文)」より
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[Presentation] A mec Hypervariable Region Sequence Typing Combined with Denaturing High-performance Liquid Chromatography (DHPLC) for Typing of Methicillin-Resistant Staphylococcus aureus (MRSA)2005
Author(s)
Yamamoto, K., Wakimoto, N., Manago, K., Kamenosono, A., Tokuda, K., Fujiyama, R., Nishi, J
Organizer
105th General Meeting of American Society for Microbiology
Place of Presentation
Atlanta, USA
Year and Date
20050606-07
Description
「研究成果報告書概要(欧文)」より
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