2006 Fiscal Year Final Research Report Summary
Investigation for tumor progression factors in pediatric lymphoma
| Project/Area Number |
17591121
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | National Research Institute for Child Health and Development |
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Principal Investigator |
NAKAGAWA Atsuko National Research Institute for Child Health and Development, Division of Pathology, Department of Clinical Laboratory Medicine, Head, 臨床検査部病理検査室, 医長 (90227736)
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| Project Period (FY) |
2005 – 2006
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| Keywords | pediatric lymphoma / Cox-2 / FISH / C-myc |
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| Research Abstract |
Newly diagnosed 133 pediatric lymphomas were classified according to the WHO classification. To classify the lymphomas, HE stain and Immunoperoxidase stainings for CD3, CD4, CD8, CD 15, CD20, CD30, CD79a, EMA, GranzymeB, and Ki-67 were performed on paraffin-embedded specimens. Detection of EBV was done by in situ hybridization (ISH) with an oligoprobe for EBV-encoded small non-polyadeneylated RNAs (EBER).
Cox-2 expression was detected by RT-PCR and immunohisochemistry. C-myc gene translocation was examined by FISH in 26 mature B-cell lymphomas (Burkitt lymphoma and diffuse large B-cell lymphoma). Nine of 10 Burkitt lymphoma cases showed c-myc gene split signals and eight cases had a t(8;14) translocation. In the diffuse large B-cell lymphoma cases, only one case out of nine cases showed c-myc gene split signals. All the 26 mature B-cell lymphomas were negative for EBER-ISH. Differentiation Burkitt lymphoma from diffuse large B-cell lymphoma is sometimes difficult by HE histology. According to the WHO classification, almost 100% (>95%) of Burkitt lymphoma cells should be positive for Ki-67. In the diffuse large B-cell lymphoma, the proliferating fraction is lower than the Burkitt lymphoma. C-myc translocation was also useful to distinguish the Burkitt lymphoma from the diffuse large B-cell lymphoma. Cox-2 expression was varied among histological types. Anaplastic large cell lymphoma (ALCL) expressed cox-2 frequently. Cox-2-positive ALCL was compared with the gene expression profiling of cox-2-negative ALCL, but no significant difference was seen. In ALCL, the expression of cyclin D3 and BCL-6 were high regardless of the cox-2 expression.
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Research Products
(12 results)
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[Journal Article] Prognostic significance of NPM-ALK fusion transcript overexpression in ALK-positive anaplastic large cell lymphoma.2007
Author(s)
Li C, Takino H, Eimoto T, Ishida T, Inagaki A, Ueda R, Suzuki R, Yoshino T, Nakagawa A, Nakamura S, Inagaki H
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Journal Title
Mod Pathol 68(Epub ahead of print)
Pages: 20
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Prognostic significance of NPM-ALK fusion transcript overexpression in ALK-positive anaplastic large cell lymphoma.
Author(s)
Li C, Takino H, Eimoto T, Ishida T, Inagaki A, Ueda R, Suzuki R, Yoshino T, Nakagawa A, Nakamura S, Inagaki H.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
