2007 Fiscal Year Final Research Report Summary
mechanism of psoriasis Neuropeptides and inflammatory cytokines enhance vascular endotherial growth factor production from epidermal keratinocytes
| Project/Area Number |
17591182
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Jichi Medical University |
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Principal Investigator |
KAKURAI Maki Jichi Medical University, School of Medicine, Assistant professor (80285791)
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| Co-Investigator(Kenkyū-buntansha) |
DEMITSU Toshio Jichi Medical University, School of Medeidne, Associate professor (20237027)
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| Project Period (FY) |
2005 – 2007
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| Keywords | VIP / neuropeptide / VEGF / psoriasis |
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| Research Abstract |
Vascular Endothelial Growth Factor (VEGF) can induce angiogenesis. VEGF has been implicated in the pathologic angiogenesis observed in psoriasis. Vasoactive intestinal peptide (VIP) has been suggested to play some roles in inflammatory dermatoses such as psoriasis. The aim of this study is to investigate the possible role of VEGF in psoriasis by analyzing epidermal VEGF production induced by VIP and cytokines. Four types of VEGF transcript, were detected in normal human epidermal keratinocytes (NHEK), and a human epidermal keratinocyte cell line DJM-1 cells, using reverse transcription-polymerase chain reaction. Next, we investigated that localization of VEGF expression in DJM-1 cells by immunohistochemistry using confocal laser scanning microscope. VEGF reactivity was found in cytoplasm of DJM-1 cells. Finally, We examined the effects of VIP and cytokines on VEGF production of DJM-1 cells using enzyme-linked immunosorbent assays. VEGF contents significantly increased in culture supernatants of DJM-1 cells treated with 1000nM VIP and/or cytokines. The combination of VIP and TNF-a induced a maximum release of VEGF ; approximately 2-fold increase compared to the control. Intercellular VEGF was determined by Western blot analysis in DJM-1 cells. The amounts of VEGF markedly increased by VIP treatment. Overall, these results suggest that several inflammatory cytokines and VIP from mast cells and nerve ending are capable of inducing VEGF production from epidermal keratiocytes, possibly leading to angiogenesis in psoriasis.
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