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2006 Fiscal Year Final Research Report Summary

THE EXPRESSION AND FUNCTION OF NOVEL GENE WITH RING H2 FINGER MOTIF IN VITRO AND IN VIVO.

Research Project

Project/Area Number 17591234
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Psychiatric science
Research InstitutionNATIONAL CENTER OF NEUROLOGY AND PSYCHIATRY

Principal Investigator

YAMADA Misa  NATIONAL CENTER OF NEUROLOGY AND PSYCHIATRY, DIVISION OF PSYCHOGERIATRICS, RISEARCHER, 精神保健研究所・老人精神保健部, 研究員 (10384182)

Co-Investigator(Kenkyū-buntansha) YAMADA Mitsuhiko  NATIONAL CENTER OF NEUROLOGY AND PSYCHIATRY, DIVISION OF PSYCHOGERIATRICS, DIRECTOR, 精神保健研究所・老人精神保健部, 部長 (60240040)
SAITO Toshikazu  SAPPORO MEDICAL UNIVERSITY, PROFESSOR, 医学部, 教授 (50128518)
Project Period (FY) 2005 – 2006
KeywordsANTIDEPRESSANT / ECT / r TMS / DIFFERENTIAL CLONING / ENDOPLASMIC RETICULUM
Research Abstract

We have previously performed EST analysis and identified some common biological changes induced after chronic antidepressant treatment as antidepressant related genes/ESTs : ADRG#1-707. In the present study, we focus on ADRG#34. The expressin of ADRG#34 was induced after chronic treatment with antidepressant, electro-convulsive treatment or repetitive transcranial magnetic stimulation in rat brain. The immunohistochemistry showed that ADRG#34 was expressed in endoplasmic reticulum but not in golgi apparatus in HEK 293 cells. Then the functions of human homologue of ADREG#34 (hADRG#34) which had RING-H2 finger motif were determined. RING finger proteins have been shown to participate in the ubiquitin-proteasome degradation system by activating E2-dependent ubiquitination. In line with this premise, we demonstrated that hADRG#34 has in vitro ubiquitination activity in the presence of ubiquitin activating enzyme (El), UbcH5b (E2), hADRG#34, ubiquitin (Ub), b-lactoglobulin as substrate, ubiquitin, and reaction buffer containing ATP. Indeed, the ubiquitinqted products immunoprecipitated by hADRG#34 were increased after addition of MG-132, proteasome inhibitor in hADRG#34 and ubiquitin co-transfected HEK293 cells. Our results indicated that ADRG#34 has E3 ubiquitin ligase activity both in vitro and in vivo.

  • Research Products

    (14 results)

All 2006 2005 Other

All Journal Article (12 results) Book (2 results)

  • [Journal Article] Induction of Neuroserpin Expression in Rat Frontal Cortex after Chronic Antidepressant Treatment and Electroconvulsive Treatment.2006

    • Author(s)
      Tanaka, et al.
    • Journal Title

      Jpn. J. Neuropsychopharmacol 26

      Pages: 51-56

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The expression of synaptic vesicle proteins after chronic antidepressant treatment in rat brain.2006

    • Author(s)
      Yamada, M, et al.
    • Journal Title

      Breathing, Feeding, and Neuroprotection.

      Pages: 82-87

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Identification of molecular systems responsible for the therapeutic effect of antidepressant.2006

    • Author(s)
      Yamada, M, et al.
    • Journal Title

      Breathing, Feeding, and Neuroprotection.

      Pages: 88-96

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Antidepressant-elicited changes in gene expression -Remodeling of neuronal circuits as a new hypothesis for drug efficacy.2005

    • Author(s)
      Yamada, et al.
    • Journal Title

      Neuropsychopharmacol Biol Psychiatry 29

      Pages: 999-1009

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Repetitive transcranial magnetic stimulation induces kf-1 expression in rat brain.2005

    • Author(s)
      Kudo, et al.
    • Journal Title

      Life Sci 76

      Pages: 2421-2429

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Ndrg2 promotes neurite outgrowth of NGF-differentiated PC12 cells2005

    • Author(s)
      Takahashi, et al.
    • Journal Title

      Neurisci. Lett 388

      Pages: 157-162

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Antidepressant-elicited changes in gene expression-Remodeling of neuronal circuits as a new hypothesis for drug efficacy.2005

    • Author(s)
      Yamada, M, et al.
    • Journal Title

      Neuropsychopharmacol. Biol. Psychiatry 29

      Pages: 999-1009

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Repetitive transcranial magnetic stimulation induces kf-1 expression in rat brain.2005

    • Author(s)
      Kudo, K, et al.
    • Journal Title

      Life Sci. 76

      Pages: 2421-2429

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Ndrg2 promotes neurite outgrowth of NGF-differentiated PC12 cells2005

    • Author(s)
      Takahashi, K, et al..
    • Journal Title

      Neurisci. Lett. 388

      Pages: 157-162

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Induction of Neuroserpin Expression in Rat Frontal Cortex after Chronic Antidepressant Treatment and Electroconvulsive Treatment.2005

    • Author(s)
      Tanaka, S, et al.
    • Journal Title

      Jpn. J. Neuropsychopharmacol. 26

      Pages: 51-56

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Olanzapine potentiates neuronal survival and neural stem cell differentiation : regulation of endoplasmic reticulum stress response proteins.

    • Author(s)
      Kurosawa, et al.
    • Journal Title

      J. Neural. Transm. (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Olanzapine potentiates neuronal survival and neural stem cell differentiation: regulation of endoplasmic reticulum stress response proteins.

    • Author(s)
      Kurosawa, S, et al.
    • Journal Title

      J. Neural. Transm. (in press.)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Breathing, Feeding, and Neuroprotection. The expression of synaptic vesicle proteins after chronic antidepressant treatment in rat brain.2006

    • Author(s)
      Yamada, et al.
    • Total Pages
      82-87
    • Publisher
      Springer-Verlag Tokyo Inc.
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Breathing, Feeding, and Neuroprotection. Identification of molecular systems responsible for the therapeutic effect of antidepressant.2006

    • Author(s)
      Yamada, et al.
    • Total Pages
      88-96
    • Publisher
      Springer-Verlag Tokyo Inc.
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2008-05-27  

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