2006 Fiscal Year Final Research Report Summary
The novel mechanism of the potentiation of estrogen action by steroid and xenobiotic receptor (SXR) in breast cancer cells.
| Project/Area Number |
17591319
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Gunma University |
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Principal Investigator |
HORIGUCHI Jun Gunma University Grad.Schl.Med., Dept. Thracic and Visceral Organ Surgery, Associate Professor, 大学院医学系研究科, 助教授 (70272242)
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| Co-Investigator(Kenkyū-buntansha) |
IWASAKI Toshiharu Gunma University Grad.Schl.Med., Dept.Integrative Physiology, Assistant Professor, 大学院医学系研究科, 講師 (80375576)
TAKESHITA Akira Toranomon Hospital, Okinaka Memorial Institute for Medical Research, Division of Endocrinology and Metabolism, 研究員 (20322646)
KOIBUCHI Yukio Gunma University Grad.Schl.Med., Dept.Thracic and Visceral Organ Surgery, Investigator, 医学部, 助手 (10323346)
KOIBUCHI Noriyuki Gunma University Grad.Schl.Med., Dept.Integrative Physiology, Professor, 大学院医学系研究科, 教授 (80234681)
IINO Yuichi Gunma University Grad.Schl.Med., Dept.Emergency Medicine, Professor, 大学院医学系研究科, 教授 (50124649)
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| Project Period (FY) |
2005 – 2006
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| Keywords | Breast cancer / SXR / ER / corepressor / SMRT |
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| Research Abstract |
Estrogen receptor (ER) is a key regulator of proliferation and differentiation in breast cancer cells. Previous studies have shown that its action may be modulated by other nuclear factors. One such candidate may be steroid and xenobiotic receptor (SXR) that is expressed in neoplastic breast tissue. In the present study, the effect of SXR on 17-estradiol (E_2)-induced transcription through ER was studied. SXR augmented ER-mediated transcription in the presence of E_2 in MCF-7 breast cancer-derived cells and CV-1 fibroblast-derived cells. On the other hand, SXR alone did not affect the estrogen response element (ERE)-containing promoter activity in CV-1 cells. SXR did not directly bind to ER nor ERE in vivo and in vitro, indicating that SXR may affect ER-mediated transcription by altering cofactor binding to ER. Although SCR did not alter the binding between ER and p300/CBP interacting protein (p/CIP), it decreased the binding of a specific corepressor, silencing mediator of retinoid and thyroid hormone receptors (SMRT) to liganded ER by mammalian two-hybrid, GST pull down and a newly developed liquid fluorescence DNA pull down assays. These results indicate that SXR augmented ER-mediated transcription by squelching SMRT. Thus, the expression of SXR in breast cancer cells may alter the ER signaling, which may play crucial role for growth and differentiation of breast cancer cells.
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[Journal Article] Diffuse sclerosing variant of papillary thyroid carcinoma : A case report.2006
Author(s)
Yoshida T, Koibuchi Y, Horiguchi J, Iijima K, Kikuchi M, Takata D, Rokutanda N, Nagaoka R, Sato A, Oyama T, Iino Y, Morishita Y
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Journal Title
Kitakanto Med J 56
Pages: 237-239
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Diffuse sclerosing variant of papillary thyroid carcinoma : A case report.2006
Author(s)
Yoshida T, Koibuchi Y, Horiguchi J, Iijima K, Kikuchi M, Takata D, Rokutanda N, Nagaoka R, Sato A, Oyama T, Iino Y, Morishita Y.
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Journal Title
Kitakanto Med. J. 56
Pages: 237-239
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Functional characterization of JMJD2A, a histone deacetylase- and retinoblastoma-binding protein2005
Author(s)
Gray SG, Iglesias AH, Lizcano F, Villanueva R, Camelo S, Jingu H, The BT, Koibuchi N, Chin WW, Kokkotou E, Dangond F
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Journal Title
J Biol Chem 280
Pages: 28507-28518
Description
「研究成果報告書概要(和文)」より
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[Journal Article] A combination phase I study of weekly paclitaxel and doxifluridine in advanced gastric cancer patients2005
Author(s)
Takeyoshi I, Makita F, Tanahashi Y, Yokomori T, Iwazaki S, Kawashima Y, Iwanami K, Yamada T, Kawate S, Hamada K, Sunose Y, Yoshida M, Horiguchi J, Iesato H, Kobayashi M, Morishita Y
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Journal Title
Anticancer Res 25
Pages: 1291-1296
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Local control by breast-conserving surgery with nipple resection2005
Author(s)
Horiauchi J, Koibuchi Y, Iijima K, Yoshida T, Takata D, Rokutanda N, Nagaoka R, Oyama T, Iino Y, Sakurai H, Morishita Y
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Journal Title
Anticancer Res 25
Pages: 2957-2959
Description
「研究成果報告書概要(和文)」より
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[Journal Article] HER2陽性転移性乳癌に対するtrastuzumabとpaclitaxel併用療法の有用性の検討2005
Author(s)
吉田 崇, 堀口 淳, 鯉淵幸生, 狩野貴之, 飯島耕太郎, 菊地麻美, 高他大輔, 六反田奈和, 長岡りん, 佐藤亜矢子, 飯野佑一, 森下靖雄
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Journal Title
Description
「研究成果報告書概要(和文)」より
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[Journal Article] HER2陽性転移性乳癌に対するtrastuzumab単独療法の有用性の検討2005
Author(s)
吉田 崇, 堀口 淳, 鯉淵幸生, 狩野貴之, 飯島耕太郎, 菊地麻美, 高他大輔, 長岡りん, 六反田奈和, 佐藤亜矢子, 飯野佑一, 森下靖雄
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Journal Title
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Functional characterization of JMJD2A, a histone deacetylase- and retinoblastoma-binding protein.2005
Author(s)
Gray SG, Iglesias AH, Lizcano F, Villanueva R, Camelo S, Jingu H, The BT, Koibuchi N, Chin WW, Kokkotou E, Dangond F.
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Journal Title
J. Biol. Chem. 280
Pages: 28507-28518
Description
「研究成果報告書概要(欧文)」より
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-
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[Journal Article] A combination phase I study of weekly paclitaxel and doxifluridine in advanced gastric cancer patients.2005
Author(s)
Takeyoshi I, Makita F, Tanahashi Y, Yokomori T, Iwazaki S, Kawashima Y, Iwanami K, Yamada T, Kawate S, Hamada K, Sunose Y, Yoshida M, Horiguchi J, Iesato H, Kobayashi M, Morishita Y.
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Journal Title
Anticancer Res. 25
Pages: 1291-1296
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Local control by breast-conserving surgery with nipple resection.2005
Author(s)
Horiguchi J, Koibuchi Y, Iijima K, Yoshida T, Takata D, Rokutada N, Nagaoka R, Oyama T, Iino Y, Sakurai H, Morishita Y.
-
Journal Title
Anticancer Res. 25
Pages: 2957-2959
Description
「研究成果報告書概要(欧文)」より
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