2006 Fiscal Year Final Research Report Summary
Analysis of H-Ras-RIN1 signaling cascade in colorectal cancer
| Project/Area Number |
17591384
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | University of Fukui |
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Principal Investigator |
GOI Takanori University of Fukui, Faculty of Medical Sciences, Assistant Professor, 医学部, 助手 (60225638)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Akio University of Fukui, Faculty of Medical Sciences, Professor, 医学部, 教授 (10174608)
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| Project Period (FY) |
2005 – 2006
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| Keywords | colorectal cancer / RIN1 gene |
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| Research Abstract |
The RIN1 gene, located on chromosome 11q13.2, is a molecule consisting of a coding region of 2352 bp, has a domain on the 3'side that binds to H-Ras protein, and is presumed to be an important molecule in an intracellular signaling pathway. Since the RIN1 molecule belonging to the effector molecules of H-Ras has not been reported in colorectal or other digestive tract cancers to date, we investigated how the RIN1 gene was involved in colorectal cancer. Fifty-two (51.5%) of 101 colorectal cancer specimens strongly expressed the RIN1 gene compared to the adjacent normal colorectal tissue. The 5-year survival rate of patients positive for the expression of the RIN1 gene was significantly poorer at 55% than that (83%) of patients negative for the expression of the RIN1 gene. Also, We confirmed that RIN1 protein was localized chiefly in the cytoplasm of colorectal cancer cell line, and bound to 14-3-3 protein, but not to Ras protein.
These results indicate that the RIN1 gene serves as an important signal transduction system for evaluating the malignancy of colorectal cancer.
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