2006 Fiscal Year Final Research Report Summary
Evaluation of effect of Ghrelin to patients who undergo surgery for upper gastrointestinal cancers and its clinical appliacation
| Project/Area Number |
17591395
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Osaka University |
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Principal Investigator |
MIYATA Hiroshi Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (80362713)
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| Co-Investigator(Kenkyū-buntansha) |
DOKI Yuichiro Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (20291445)
YASUDA Takushi Kinki University, Graduate School of Medicine, Associate Professor, 医学部, 助教授 (10324782)
FUJIWARA Yoshiyuki Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (40314330)
TAKIGUICHI Syuji Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (00301268)
KANGAWA Kenji National Cardiovascular Center Research Institute, Deputy Director, 生化学部, 部長(研究職) (00112417)
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| Project Period (FY) |
2005 – 2006
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| Keywords | Ghrelin / esophagectomy / gastrectom / body weight loss / feeding center |
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| Research Abstract |
1 In order to evaluate the effect of Ghrelin to proliferation of cancer cells, we performed MTT assay and counted the number of cancer cells after adding Ghrelin to culture medium of esophageal cancer cell lines (TE3,TE8) and gastric cancer cell lines (MKN-45,KATO-III), and found that Ghrelin did not affect proliferation of cancer cells. This result confirmed the safety of administering Ghrelin to patients with uppergastrointestinal cancers after surgery.
2 We administered Ghrelin to three healthy donors and also did saline as control to three healthy donors immediately after orally taking nutrition liquid (Racol), and examined Ghrelin, growth hormone and insulin levels in serum and blood glucose levels. We also examined gastric emptying function using breath test of measuring 13-C acetate. As a result, we found that serum growth hormone significantly increased as serum Ghrelin increased in Ghrelin group compared with control group and that serum insulin and blood glucose increased more immediately in Ghrelin group than control group. We also found that gastric emptying time shortened in Grhelin group. These results indicated that administrating Ghrelin to healthy donors facilitates gastric emptying function and glucose absorption.
3 After a phase II study of administrating Ghrelin to patients who undergo gastrectomy for gastric cancers was approved by the ethics committees at Osaka University Hospital, ten patients were enrolled in this study between August 2006 and March 2007. Ghrelin at a dose of 3μ g/kg were administrated to patients twice a day via drip infusion on days 5 to 15 after surgery, and we evaluated oral intake, intestinal motility, absorption function of small intestine and change of various blood sample data, such as growth hormone, albumin ghrelin and leptin. We plan to analyze patients data obtained from this study in detail.
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Research Products
(3 results)
