2006 Fiscal Year Final Research Report Summary
補助人工心臓下の虚血性心筋症に対する平滑筋細胞移植の効果
| Project/Area Number |
17591463
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
YASUDA Tamotsu Tokyo Medical University, Medicine, Associate Professor, 医学部, 助教授 (80324119)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Go Tokyo Medical University, Medicine, Professor, 医学部, 兼任教授 (60242492)
TOMITA Shigeyuki Kanazawa University Hospital, Instructor, 助手 (90334771)
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| Project Period (FY) |
2005 – 2006
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| Keywords | cell transplantation / regenerative medicine / cardiac transplantation / Y-chromosome |
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| Research Abstract |
Syngeneic Lwis rats were used for this study. First, left anterior descending artery was ligated using 6-0 Prolene to obtain anterior myocardial infarction in the female rats. After this procedure, echocardiography was repeated to examine cardiac function. 4 weeks after myocardial infarction, echocardiography was done, and if 25%of myocardium was infarcted, that rat went to next step. Under general anesthesia, infarcted hearts were extirpated as donor heart, which was then implanted into recipient abdomen, where ascending aorta and pulmonary artery of donor were anastomosed to abdominal aorta and inferior vena cava using 9-0 Prolene. Simultaneously,, smooth muscle cells were transplanted in group T. 4weeks after heterotopic transplantation, hearts were excised in order to measure cardiac function with langendorff apparatus, to count transplanted male cells, and to do histopathological examination.
In Langendorff study, hearts in group T showed better cardiac function than those in group C (control) significantly. In addition, the percentage of male smooth muscle cells transplanted into infarcted myocardium was about 15%. One of the reasons why cardiac function in group T was better than that in group C was that the number of artrioles in group T was higher than that in group C, so it has good impact on cardiac function.
In conclusion, cell transplantation in infarcted myocardium could be effective in terms of cardiac function. This concept might be applied to clinical settings, such as whether or not the patients who suffered from heart failure, can wean from LVAD implantation after smooth muscle cell transplantation.
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