2006 Fiscal Year Final Research Report Summary
Development of Tissue Engineered Heart Valve utilizing Autologous Bone Marrow Cells and Decellularized Homograft
| Project/Area Number |
17591489
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
SAITO Satoshi Tokyo Women's Medical University, School of Medicine, Associate Professor, 医学部, 講師 (60246551)
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| Co-Investigator(Kenkyū-buntansha) |
SHIN'OKA Toshiharu Tokyo Women's Medical University, School of Medicine, Assistant Professor, 医学部, 助教授 (20192122)
KUROSAWA Hiromi Tokyo Women's Medical University, School of Medicine, Professor, 医学部, 教授 (50075511)
ISHIYAMA Masakuni Tokyo Women's Medical University, School of Medicine, Assistant, 医学部, 助手 (30246558)
YAMAMOTO Noboru Tokyo Women's Medical University, School of Medicine, Assistant, 医学部, 助手 (50297470)
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| Project Period (FY) |
2005 – 2006
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| Keywords | Regenerative Medicine / Tissue Engineering / Tissue Engineered graft / Biodegradable Scaffold / Bone Marrow Cells / Decellularization / Homograft / Tissue Engineered valve |
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| Research Abstract |
Although in the hope of being the main resources of matrix for clinical application, pig aorta which is of high thickness have not been decellularized efficiently so far. Therefore the optimized decellularization protocol for pig aorta in this study based on Courtman's protocol was developed. DNA content test, Hematoxylin and eosin (HE) staining and immunohistological examination against a-actin, vimentin and desmin demonstrated the high decellularizaiton efficiency of the protocol. The high efficiency in cell removal didn't compromise the strength of the matrix which maintained mechanical strength comparable to that of the matrix from Courtman's protocol. Less immunoreaction and calcification in the matrix retrieved from subcutaneous model further proved the higher decellularization efficiency of the optimized protocol compare to the Courtman's protocol. In conclusion, we think the decellularization protocol present in this study is an ideal decellularization protocol for pig aorta.
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