2006 Fiscal Year Final Research Report Summary
A Novel Strategy against Methicillin-resistant Staphylococcus Aureus graft infection by using Sustained-release Vancomycin
| Project/Area Number |
17591494
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Kinki University |
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Principal Investigator |
KANEDA Kouzou Kinki Univeristy, Hospital, Assistant Professor, 医学部附属病院, 講師 (40340830)
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| Co-Investigator(Kenkyū-buntansha) |
IKEDA Tadashi Kyoto University, Graduate School of Medicine, Associate professor, 医学研究科, 助教授 (40281092)
MARUI Akira Kyoto University, Graduate School of Medicine, Assistant Professor, 医学研究科, 助手 (60402856)
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| Project Period (FY) |
2005 – 2006
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| Keywords | Vancomycin / MRSA / Graft infection / PLCA / sustained-release sheet / sepsis |
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| Research Abstract |
Introduction : MRSA graft infection is most serious complications after vascular surgery. VCM is a potent antibiotic to MRSA; however, systemic administration of VCM is not so effective in MRSA graft infection. Therefore we investigated whether a local sustained-release of VCM prevents MRSA graft infection.
Methods : We have developed poly-L-lactide-co-caprolactone (PLCA) sheet that enabled sustained release of VCM for 2 weeks. An e-PTFE vascular graft patch (1.5 mm^2) was sutured at the anterior wall of the incised aorta of a rat. MRSA (1.0×10^3 colony forming units; CFU) was inoculated onto the graft surface. Thereafter, the graft was treated as follows (n=6 each) : no treatment (Control group), an aqueous solution of VCM (VCM/solution group), PLCA-containing VCM (VCM/PLCA group). After seven days, the graft and blood were sampled and cultured.
Results : The MRSA count in the graft of VCM/PLCA group were significantly lower than those of the other groups. Blood cultures of VCM/PLCA group were all negative, whereas those of the other groups were all positive. The survival rate in VCM/PLCA group at 28days was higher than Control group (83.3% vs 16.7%).
Conclusion : A local sustained-release sheet incorporating VCM reduced MRSA in the infected vascular graft, prevented sepsis, and drastically improved survival. This method may develop more effective but less invasive adjuvant for prevention of prosthetic MRSA graft infection.
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